Anti-Excision Repair Cross Complement (ERCC2). IgG fraction (monoclonal)

IgG fraction from mouse monoclonal antibody (clone 2A10/E2) obtained by immunization of mice with a peptide of ERCC2 (80 kDa subunit of TFIIH). The IgG fraction was purified using Protein G-Sepharose.

• Storage: Store at -20°C.
• Stability: Stable for at least one year. Avoid repeated freezing and thawing.
• Biological activity: This antibody can be used for ELISA assay (1:8,000 dilution). It recognizes ERCC2 subunit of TFIIH and it has not cross reaction with ERCC3 subunit. ERCC2 is an ATP-dependent 5'-3' DNA helicase, component of the core-TFIIH transcription factor. It is involved in nucleotide excision repair of DNA by opening around the damage, and the initiation of transcription catalyzed by RNA polymerase II.
• Formulation: Solution at 0.5 mg/mL in PBS
• References: Schultz, P. et al. (2000) Cell 105, 599 - 607.
  Reardon, J.T. et al. (1996) Proc. Natl. Acad. Sci. USA 93 6482-
  6487.
  Drapkin, R. et al (1994) Nature 368, 769 - 772.

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