Rabbit anti-Human Survivin IgG fraction polyclonal antibody

IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (EETAKKVRRAIEQLAAMD) corresponding to amino acids 125-142 of human Survivin. The IgG fraction was purified using Protein G-Sepharose.

• Storage: Store at -20°C.
• Stability: Stable at least one year at -20°C. Avoid repeated freezing and thawing.
• Biological activity: This polyclonal antibody reacts in ELISA (1: 50 dilution) and Western blot (1: 1,000) using the immunizing peptide. Survivin is a 16 kDa apoptosis inhibitor protein (IAP). It binds and inhibits the caspase-3 proteolytic activity and controls the checkpoint in the G2/M phase of the cell cycle to promote cell division by the control of microtubule stability and assembly of a normal mitotic spindle. Survivin is highly expressed during fetal development, transformed cell lines, human cancers and non-Hodgkin's lymphomas.
• Formulation: Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
• References: Ambrosini, G. et al. (1997) Nat. Med. 3, 917-921. Li, F. et al. (1998) Nature 396, 580-584. Verhagen, A.M. et al. (2001) Genome Biol. 2, 3009.1-3009.10. Giodini, A. et al. (2002) Cancer Res. 62, 2462-2467. Ryan, B.M. et al. (2006) Ann. Oncol. 17, 597-604.

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