Rabbit anti-Human Cullin7 (CUL 7) IgG fraction polyclonal antibody

IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (RGVPYASCTATQSFSTF) corresponding to amino acids 1681-1697 of human Cullin 7. The IgG fraction was purified using Protein G-Sepharose.

• Storage: Store at -20°C.
• Stability: Stable at least one year at -20°C. Avoid repeated freezing and thawing.
• Biological activity: This polyclonal antibody reacts in ELISA (1: 100 dilution) and Western blot (1: 1,000 dilution) using the immunizing peptide. Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 7 (CUL 7) is a component of SCF-like E3 ubiquitin ligase complex and may play a role in the degradation of proteins involved in endothelial proliferation and/or differentiation. Cullin 7 is a new oncogene that cooperates with Myc in transformation by blocking Myc-induced apoptosis in a p53-dependent manner.
• Formulation: Solution at 0.5 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
• References: Dias, D.C. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 16601-16606. Kasper, J.S. et al. (2006) Biochem. Biophys. Res. Commun. 348, 132-138. Kim, S.S. et al. (2007) Cancer Res. 67, 9616-9622.

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