Acidic Fibroblast Growth Factor (FGF 1), Human Recombinant

Acidic FGF is a mitogen for cells of mesodermal and neuroectodermal origin, including fibroblasts, endothelial cells, astrocytes, neuroblasts, osteoblasts, and smooth muscle cells. This protein is composed of 140 amino acid residues, and has a 55% homology with basic FGF, including two conserved cysteine residues. Human Acidic FGF is produced by genetically engineered yeast, and purified by heparin-Sepharose affinity chromatography.

• Molecular weight: 15.5 kDa
• Purity: Over 95% pure by HPLC analysis and SDS gel electrophoresis.
• Storage: Store at -80°C. It is also possible to keep this protein at low pH (2-5) for a few days.
• Stability: Unstable without a carrier protein like gelatin or bovine serum albumin. It is recommended to add some of these proteins, prior to aliquoting, for longer storage.
• Biological activity: Human acidic FGF is mitogenic for a variety of normal cells in culture including fibroblasts, glial cells, vascular endothelial cells.
• Formulation: 10 mM Tris-HCl, 1.5 M NaCl, pH 7.0 Concentration: 0.50 mg protein/ml.
• References:
  
  1. Gimenez-Gallego et al., Biochem. Biophys. Res. Comm.(1986) 138:611
  2. Thornton et al. (1983), Science 222, 623
  3. Gospadorowicz, D. (1987) Methods Enzymol. 147:106

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