Austral Biologicals GF-030 - Basic Fibroblast Growth Factor (FGF 2) human (RECOMBINANT), 10 µg

Basic Fibroblast Growth Factor (FGF 2), Human Recombinant

10 µg
$50.00
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Austral Biologicals GF-030 - Basic Fibroblast Growth Factor (FGF 2) human (RECOMBINANT), 10 µg
  • Austral Biologicals GF-030 - Basic Fibroblast Growth Factor (FGF 2) human (RECOMBINANT), 10 µg
  • Austral Biologicals GF-030 - Basic Fibroblast Growth Factor (FGF 2) human (RECOMBINANT), 10 µg
Basic Fibroblast Growth Factor (FGF 2), Human Recombinant

Basic Fibroblast Growth Factor (FGF 2), Human Recombinant

Catalog no. GF-030-3
$50.00
Size

Human Basic Fibroblast Growth Factor is a mitogen that stimulates cell growth for such cells as fibroblasts, endothelial cells, myoblasts, glial cells and smooth muscle cells. Basic FGF is composed of 146 amino acid residues (pro 1 to ser 146), and is 55% homologous with acidic FGF, including two conserved cysteine residues. Human basic FGF is produced by genetically engineered yeast, and purified by heparin-Sepharose affinity chromatography.

  • Molecular weight: 17.5 kDa
  • Purity: Over 95% pure by N-terminal amino acid sequencing, amino acid composition, HPLC analysis, and SDS gel electrophoresis.
  • Storage: Store at +4°C. Reconstitution is best in water or buffer near neutral pH, since it is unstable in acidic solutions. After reconstitution, storage in aliquots at -80°C is recommended.
  • Stability: Several months, lyophilized or in solution, at pH 6-7 and +4°C.
  • Biological activity: The mitogenic activity of human basic FGF is identical to natural bovine basic FGF, as measured by stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells.
  • Formulation: Lyophilized powder
  • References:

    1. Gospodarowicz, D. (1975) J. Biol. Chem. 250, 2515-2520.
    2. Folkman, J. and Klagsbrun, M. (1987) Science 235,442-447
    3. Fox, J., et al. (1996) Journal of Biological Chemistry 271: 12578-12584.

Publications using Austral Biologicals GF-030

The following scientific publications report use of Austral Biologicals GF-030:

  1. Cell-cultured PDMS vascular model to allow placement of implant devices. Okuno et al., BMC Methods 3:16 (2026).
  2. Geometrically engineered organoid units and their assembly for pre-construction of organ structures. Kadotani et al., APL Bioengineering (2024).
  3. Hydrolytic hydrogels tune mesenchymal stem cell persistence and immunomodulation for enhanced diabetic cutaneous wound healing. Martin et al., Biomaterials (2023).
  4. Migration of endothelial cells on the surface of anodized Ni-Ti stent strut. Wang et al., Frontiers in Medical Technology (2023).
  5. Host type 2 immune response to xenogeneic serum components impairs biomaterial-directed osteo-regenerative therapies. Martin et al., Biomaterials (2022).
  6. Antiangiogenic Activity of Flavonols in Chorioallantoic Membrane (CAM) Assay. Okamura et al., Food Science and Technology Research 26:891-896 (2020).
  7. Hydrostatic pressure promotes endothelial tube formation through aquaporin 1 and Ras-ERK signaling. Yoshino et al., Communications Biology (2020).
  8. YAP and TAZ limit cytoskeletal and focal adhesion maturation to enable persistent cell motility. Mason et al., Journal of Cell Biology (2019).
  9. Fluid shear stress suppresses ICAM-1-mediated transendothelial migration of leukocytes in coculture model. Sakamoto et al., Biochemical and Biophysical Research Communications (2018).
  10. Skeletal cell YAP and TAZ combinatorially promote bone development. Kegelman et al., FASEB Journal (2018).
  11. A Novel Technique for Accelerated Culture of Murine Mesenchymal Stem Cells that Allows for Sustained Multipotency. Caroti et al., Scientific Reports 7:13334 (2017).
  12. Proliferation-Related Activity in Endothelial Cells Is Enhanced by Micropower Plasma. Suzuki and Yoshino, BioMed Research International (2016).
  13. Endothelial Cell Response Under Hydrostatic Pressure Condition Mimicking Pressure Therapy. Yoshino et al., Cellular and Molecular Bioengineering 8:296-303 (2015).
  14. Basic fibroblast growth factor predicts cardiovascular disease occurrence in participants from the Veterans Affairs Diabetes Trial. Zimering et al., Frontiers in Endocrinology 4:183 (2013).
  15. Haemodynamically dependent valvulogenesis of zebrafish heart is mediated by flow-dependent expression of miR-21. Banjo et al., Nature Communications 4:1978 (2013).
  16. Role of nesprin-1 in nuclear deformation in endothelial cells under static and uniaxial stretching conditions. Anno et al., Biochemical and Biophysical Research Communications (2012).
  17. Role of paxillin in the early phase of orientation of vascular endothelial cells exposed to cyclic stretching. Huang et al., Biochemical and Biophysical Research Communications (2012).
  18. Brazilian Propolis Suppresses Angiogenesis by Inducing Apoptosis in Tube-Forming Endothelial Cells through Inactivation of Survival Signal ERK1/2. Kunimasa et al., Evidence-Based Complementary and Alternative Medicine (2011).
  19. Cyclic Force Applied to FAs Induces Actin Recruitment Depending on the Dynamic Loading Pattern. Ueki et al., Open Biomedical Engineering Journal 4:129-134 (2010).
  20. Role of p120-catenin in the morphological changes of endothelial cells exposed to fluid shear stress. Sakamoto et al., Biochemical and Biophysical Research Communications (2010).
  21. Measurements of strain on single stress fibers in living endothelial cells induced by fluid shear stress. Ueki et al., Biochemical and Biophysical Research Communications (2010).
  22. Effect of spatial gradient in fluid shear stress on morphological changes in endothelial cells in response to flow. Sakamoto et al., Biochemical and Biophysical Research Communications (2010).
  23. Direct measurement of shear strain in adherent vascular endothelial cells exposed to fluid shear stress. Ueki et al., Biochemical and Biophysical Research Communications (2010).
  24. Ex vivo enrichment of mesenchymal cell progenitors by fibroblast growth factor 2. Bianchi et al., Experimental Cell Research (2003).
  25. Resveratrol and quercetin inhibit angiogenesis in vitro. Igura et al., Cancer Letters (2001).
  26. Transcriptional regulation of connective tissue growth factor by cortisol in osteoblasts. Pereira et al., American Journal of Physiology-Endocrinology and Metabolism 279:E570-E576 (2000).
  27. Proliferation kinetics and differentiation potential of ex vivo expanded human bone marrow stromal cells: implications for their use in cell therapy. Banfi et al., Experimental Hematology (2000).
  28. Placental lactogen-I gene activation in differentiating trophoblast cells: extrinsic and intrinsic regulation involving mitogen-activated protein kinase signaling pathways. Peters et al., Journal of Endocrinology 165:443-456 (2000).
  29. Ras/MEK/ERK Up-regulation of the Fibroblast KCa Channel FIK Is a Common Mechanism for Basic Fibroblast Growth Factor and Transforming Growth Factor-β Suppression of Myogenesis. Peña et al., Journal of Biological Chemistry 275:13677-13682 (2000).
  30. Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model. Muraglia et al., Journal of Cell Science 113:1161-1166 (2000).
  31. Bone morphogenetic proteins induce the expression of noggin, which limits their activity in cultured rat osteoblasts. Gazzerro et al., Journal of Clinical Investigation 102:2106-2114 (1998).
  32. A Nude Mouse Model for Human Bone Formation in Unloaded Conditions. Muraglia et al., Bone (1998).
  33. Insulin-related growth factors stimulate proliferation of retinal progenitors in the goldfish. Boucher and Hitchcock, Journal of Comparative Neurology 394:386-394 (1998).
  34. Mitogen-Activated Protein Kinase Kinase (MEK) Activity Is Required for Inhibition of Skeletal Muscle Differentiation by Insulin-Like Growth Factor 1 or Fibroblast Growth Factor 2. Weyman and Wolfman, Endocrinology 139:1794-1800 (1998).
  35. Ets-1 is an early response gene activated by ET-1 and PDGF-BB in vascular smooth muscle cells. Naito et al., American Journal of Physiology-Cell Physiology 274:C472-C480 (1998).
  36. Fibroblast growth factor-2 supports ex vivo expansion and maintenance of osteogenic precursors from human bone marrow. Martin et al., Endocrinology 138:4456-4462 (1997).
  37. Differential responses of scirrhous and well-differentiated gastric cancer cells to orthotopic fibroblasts. Yashiro et al., British Journal of Cancer 74:1096-1103 (1996).
  38. Pro-inflammatory cytokines downregulate platelet derived growth factor-α receptor gene expression in human osteoblastic cells. Köse et al., Journal of Cellular Physiology 166:188-197 (1996).

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