Anti-Infectious Salmon Anemia Virus (ISAV) Matrix Protein, IgG Fraction, Monoclonal, Clone 1D6/C8

Mouse monoclonal antibody IgG fraction (clone 1D6/C8) obtained by immunizing mice with a purified matrix recombinant protein of ISAV. The IgG fraction was purified using Protein G-Sepharose.

• Storage: Store at -20°C.
• Stability: Stable at least one year at -20°C. Avoid repeated freezing and thawing.
• Biological activity: This antibody can be used in ELISA (1:500 dilution), Western blot (1:500 dilution), and indirect immunofluorescence (1:250). It recognizes 23 kDa protein corresponding to ISAV matrix protein. ISAV is an enveloped virus that belongs to the family Orthomyxoviridae and genus Isavirus. Its genoma comprises eight negative-sense, single-stranded RNA segments and encodes four major structural proteins including the matrix, the nucleoprotein, and two membrane glycoproteins (hemagglutinin-esterase and gp50). ISAV infects mainly endothelial cells and leukocytes and causes a multisystemic disease characterized by high mortality with exophthalmia, pale gills, ascites, hemorrhagic liver necrosis, and renal interstitial hemorrhage.
• Formulation: Solution at 1.0 mg/mL in PBS.
• References:
  
  1. Rimstad, E. & Mjaaland, S. (2002) APMIS 110, 273-282.
  2. Ritchie, R.J. et al. (2002) Virus Res.84, 161-170.
  3. Falk,K. et al. (2004) J. Virol. 78, 3063-3071.
  4. Kibenge, F.S. et al. (2007) 4, 34.
  5. Goic, B. et al. (2008) Virology 379, 55-63.

Download technical datasheet (PDF)