Anti-Infectious Salmon Anemia Virus Nucleoprotein (ISAV), IgG Fraction, Monoclonal, Clone 2C7/E12

Mouse monoclonal antibody IgG fraction (clone 2C7/E12) obtained by immunizing mice with a purified C-terminal ISAV nucleoprotein recombinant fragment (aa161-616). The IgG fraction was purified using Protein G-Sepharose.

• Storage: Store at -20°C.
• Stability: Stable at least one year at -20°C. Avoid repeated freezing and thawing.
• Biological activity: This antibody can be used in ELISA (1:2,000 dilution), Western blot (1:1,000 dilution), immunoprecipitation (1:100 dilution), and indirect immunofluorescence (1:500). It recognizes a 68 kDa protein corresponding to ISAV nucleoprotein. ISAV is an enveloped virus that belongs to the family Orthomyxoviridae and genus Isavirus. Its genoma comprises eight negative-sense, single-stranded RNA segments and encodes four major structural proteins including the matrix, the nucleoprotein, and two membrane glycoproteins (hemagglutinin-esterase and gp50). ISAV infects mainly endothelial cells and leukocytes and causes a multisystemic disease characterized by high mortality with exophthalmia, pale gills, ascites, hemorrhagic liver necrosis, and renal interstitial hemorrhage.
• Formulation: Solution at 1.0 mg/mL in PBS.
• References:
  
  1. Rimstad, E. & Mjaaland, S. (2002) APMIS 110, 273-282.
  2. Falk,K. et al. (2004) J. Virol. 78, 3063-3071.
  3. Aspehaug, V. et al. (2004) Virus Res. 106, 51-60.
  4. Goic, B. et al. (2008) Virology 379, 55-63.

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