Rabbit anti-Alfalfa Mosaic Virus (AMV) IgG fraction POLYCLONAL

Rabbit polyclonal antibody IgG fraction obtained by immunizing rabbits with a mix of two synthetic peptides corresponding to aa14-27 (KPTKRSQNYAALRK), and aa193-205 (ERKAFPRAGPLRS) of coat protein from AMV. The IgG fraction was purified using Protein G-Sepharose.

• Storage: Store at -20°C.
• Stability: Stable at least one year at -20°C. Avoid repeated freezing and thawing.
• Biological activity: This polyclonal antibody reacts with extracts of AMV-infected plants in PTA (Plate-Trapped Antigen)-ELISA and Western Blot assays (1: 50 dilution). AMV is a positive-strand RNA virus with tripartite genome belonging to the family Bromoviridae. It forms bacilliform or spherical particles and is transmitted in a non-persistent manner by numerous aphids species. The AMV genome is encapsidated by a single coat protein (24 kDa) which plays a key role in early and late AMV infection, functioning in genome activation, RNA replication, and in cell-to-cell systemic movement of virus.
• Formulation: Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
• References: Herranz, M.C. et al. (2012) Mol. Plant Microbe Interact. 25, 1093-1103.
  Bol, J.F. (2005) Annu. Rev. Phytopathol. 43, 39 - 62.
  Bol, J.F (2003) Mol. Plant Pathol. 4, 1 - 8.

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