Rabbit Anti-Grapevine Virus A (GVA) IgG fraction POLYCLONAL

IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide corresponding to aa21-35 (KAQPTDDASESGYDR) of coat protein from GVA. The IgG fraction was purified using Protein G-Sepharose.

• Storage: Store at -20°C.
• Stability: Stable at least one year at -20°C. Avoid repeated freezing and thawing.
• Biological activity: This polyclonal antibody reacts in ELISA and Western Blot assays (1: 50 dilution) using the corresponding peptide. GVA belongs to the genus Vitivirus of the family Flexiviridae and its genome is composed by a single-stranded positive-sense RNA and a single coat protein subunit of 21 - 23 kDa. Mutations in the coat protein gene indicated that this structural protein is also required for cell-to-cell movement of the virus. GVA is transmitted by insect vectors or grafting.
• Formulation: Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
• References: du Preez, J. (2011) Arch. Virol. 156, 1495-14503
  Galiakparov, N. et al. (2003) Virology 306, 42-50.
  Goszczynski, N. & Jooste, A.E.C. (2003) Eur. J. Plant Pathol. 109, 397-403.
  Minafra, A. et al. (1997) Arch. Virol. 142, 417-423.

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