Rabbit Anti-Grapevine Fanleaf Virus (GFLV) Coat Protein (Peptides) IgG Fraction POLYCLONAL

Rabbit polyclonal antibody IgG fraction obtained by immunizing rabbits with a mix of six synthetic peptides corresponding to aa11-19 (PKDCQANRY), aa71- 81 (DAYNRITSRITK), aa261-270 (DWNELFKYPG), aa282-293 (KIRSPYHRTPAR), aa360-370 (REEIEIGSRFFK), and aa405-16 (QWSLNTEFGKKSS) of coat protein from GFLV. The IgG fraction was purified using Protein G-Sepharose.

• Storage: Store at -20°C.
• Stability: Stable at least one year at -20°C. Avoid repeated freezing and thawing.
• Biological activity: This polyclonal antibody reacts with extracts of GFLV-infected plants in PTA (Plate-Trapped Antigen)-ELISA and Western blot (1: 1,000 dilution). GFLV belongs to the genus Nepovirus of the family Comoviridae and its genome is composed by two single-stranded positive-sense RNAs.It has a single coat protein with a molecular weight of 56 kDa, this protein has been reported to be involved in the specific transmission of the GFLV by its nematode vector Xiphinema index.
• Formulation: Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
• References: Schellenberger, P. et al. (2011) PloS Pathogens 7 (5), e1002034.
  Wetzel, T. et al. (2001) Virus Res. 75, 139-145.
  Serghini, M. A. et al. (1990) J. Gen. Virol. 71, 1433-1441.

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