Anti-Vibrio Cholerae 01, IgG Fraction, Monoclonal
Anti-Vibrio Cholerae 01, IgG Fraction, Monoclonal
Mouse IgG anti Vibrio choleral monoclonal antibody reacting with LPS epitopes present in the bacterial cell wall. The antigen used for immunization corresponds to Vibrio cholerae 01, serotype Ogawa, biotype ELT or, inactivated at 56°C for 30 min. This antibody performs well in ELISA, IFI, Western blots and particle aglutination assays. Evaluated through ELISA and indirect immuno-fluorescence (IFI) with two panels of microorganisms normally present in stool samples. One panel include 72 potential antigenically related bacterial strains, the second panel included pathogenic bacterial strains involved in diarrhea cases.
- Storage: Store at +4°C. May be frozen at -20°C.
- Stability: At least 1 year +4°C.
- Biological activity: The antibody reacts with cholera antigens in Western blots.
- Formulation: Solution at 0.25 mg/mL in 0.1M Tris-HCl (pH 7.5)
-
References:
1. Development of highly specific monoclonal antibodies for the diagnosis of Vibrio cholerae 0 (1995) Hybridoma 14(3) 277-278. Castillo L., Castillo D., Silva W., 2. Zapapta L., Reiod M., Ulloa MT., Maldonado A., Valenzuela ME., Bustos R., Tamplin ML., Martinez MA., De Ioannes AE., Jamett A., Yudelevich A., Becker MI.
Published product use
A peer-reviewed study reported use of VCM-5261-5 for Vibrio cholerae O1 tissue staining:
- Confocal immunostaining: VCM-5261-5 was used at 2.5 µg/mL to stain V. cholerae O1 in infected rabbit ileal-loop tissue for scanning confocal microscopy. Nielsen et al., PLOS Pathogens (2010).