{"product_id":"tm-901z","title":"Anti-human Mediator 10 (MED10 or HNUT2) IgG fraction (monoclonal)","description":"\u003cp\u003eIgG fraction from mouse monoclonal antibody (clone 8A1\/F4) obtained by immunization of mice with human MED10 (25 kDa). The IgG fraction was purified using Protein G-Sepharose.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStability:\u003c\/strong\u003e Stable for at least one year. Avoid repeated freezing and thawing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological activity:\u003c\/strong\u003e This antibody can be used in ELISA assays (1: 8,000 dilution) and Western blot. It recognizes human MED10 (hNut2), a subunit protein of Mediator (MED) complex. The Mediator is a multiprotein coactivator required for activation of RNA polymerase II transcription by DNA binding transcription factors. The MED10 has been assigned to the middle domain of Mediator and it interacts physically with the carboxy-terminal domain (CTD) of largest RNA polymerase II subunit according to electron microscopic data.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Solution at 1.0 mg\/mL in PBS\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReferences:\u003c\/strong\u003e Guglielmi, B. al (2004) Nucleic Acids Res. 32, 5379 – 5391; Bourbon, H-M. et al (2004) Mol Cell 14, 553 – 557; Lewis, B. \u0026amp; Reinberg, D. (2003) J. Cell Sci. 116, 3667 – 3675; Boube, M. et al (2002) Cell 110, 143 – 151; Woychik, N. \u0026amp; Hampsey, M. (2002) Cell 108, 453 – 463.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp class=\"ab-datasheet-link\"\u003e\u003ca href=\"https:\/\/helix-chartreuse-ypja.squarespace.com\/s\/Austral_Biologicals_TM-901Z_Datasheet.pdf\" target=\"_blank\" rel=\"noopener\"\u003e\u003cstrong\u003eDownload technical datasheet (PDF)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Austral Biologicals","offers":[{"title":"150 µg","offer_id":52518885490990,"sku":"TM-901Z-55","price":425.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1006\/2846\/3918\/files\/austral-biologicals-tm-901z-150ug.png?v=1788007708","url":"https:\/\/www.australbiologicals.com\/products\/tm-901z","provider":"Austral Biologicals","version":"1.0","type":"link"}