{"product_id":"tm-501","title":"Anti- human Sirtuin type 1 (SIRT 1) IgG fraction (monoclonal)","description":"\u003cp\u003eIgG fraction from mouse monoclonal antibody (clone 3H2\/G8) obtained by immunization using an ovalbumin-conjugated synthetic peptide corresponding to aa551–aa599 of human silent information regulator 2 homolog (Sirt1) protein. The IgG fraction was purified using Protein G-Sepharose.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStability:\u003c\/strong\u003e Stable for at least one year. Avoid repeated freezing and thawing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological activity:\u003c\/strong\u003e This antibody can be used for ELISA assay (1: 8,000 dilution), immunoblot and immunoprecipitation analysis. It recognizes Sirt1 (120kDa), a NAD-dependent p53 deacetylase; protein involved in diverse processes ranging from regulation of gene silencing to DNA damage response in mammalian cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Solution at 1.0 mg\/mL in PBS (pH 7.2).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReferences:\u003c\/strong\u003e Langley, E. et al. (2002) EMBO J. 21, 2383 – 2396.\u003cbr\u003eVaziri, H. et al. (2001) Cell 107, 149 – 159.\u003cbr\u003eFrye, R. (1999) Biochem. Biophys. Res. Commun. 260, 273 – 279.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp class=\"ab-datasheet-link\"\u003e\u003ca href=\"https:\/\/helix-chartreuse-ypja.squarespace.com\/s\/Austral_Biologicals_TM-501_Datasheet.pdf\" target=\"_blank\" rel=\"noopener\"\u003e\u003cstrong\u003eDownload technical datasheet (PDF)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Austral Biologicals","offers":[{"title":"150 µg","offer_id":52518884966702,"sku":"TM-501-55","price":425.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1006\/2846\/3918\/files\/austral-biologicals-tm-501-150ug.png?v=1788007913","url":"https:\/\/www.australbiologicals.com\/products\/tm-501","provider":"Austral Biologicals","version":"1.0","type":"link"}