{"product_id":"tm-1411w","title":"Anti-human Di\/Tri-Methyl K27 Histone h3 (DI\/TRI-Methylated H3 K27) IgG fraction","description":"\u003cp\u003eIgG fraction from mouse monoclonal antibody (clone 7B11\/G5) obtained by immunization of mice with a KLH-conjugated synthetic peptide TKAAR[dimethyl-K]SAP-C corresponding to aa22 – aa30 of human histone H3. The IgG fraction was purified using Protein G-Sepharose.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStability:\u003c\/strong\u003e Stable for at least one year. Avoid repeated freezing and thawing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological activity:\u003c\/strong\u003e This antibody can be used in ELISA assays (1: 1,000 dilution) and immunofluorescence assays. It recognizes di- and trimethylated H3 K27. The methylation of specific histone lysines controls aspects of short- and long-term transcriptional regulation, in addition to the propagation of bulk chromosome structure and stability. Methylated histone H3 K27 is also involved in transcriptional silencing of human polycomb target genes and earliest chromatin changes in the X-chromosome inactivation. Methylated histone H3 K27 is considered a mark of repressive chromatin states\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Solution at 1.0 mg\/mL in PBS\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReferences:\u003c\/strong\u003e Hampsey, M \u0026amp; Reinberg, D. (2003) Cell 113, 429 – 432; Rougeulle, C. et al. (2004) Mol. Cell. Biol. 24, 5475 – 5484; Kirmizis, A. et al. (2004) Genes \u0026amp; Dev. 18, 1592 – 1605; Sims, R. J. et al. (2003) Trends Genet. 19, 629 – 639.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp class=\"ab-datasheet-link\"\u003e\u003ca href=\"https:\/\/helix-chartreuse-ypja.squarespace.com\/s\/Austral_Biologicals_TM-1411W_Datasheet.pdf\" target=\"_blank\" rel=\"noopener\"\u003e\u003cstrong\u003eDownload technical datasheet (PDF)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Austral Biologicals","offers":[{"title":"150 µg","offer_id":52518884213038,"sku":"TM-1411W-55","price":425.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1006\/2846\/3918\/files\/austral-biologicals-tm-1411w-150ug.png?v=1788007966","url":"https:\/\/www.australbiologicals.com\/products\/tm-1411w","provider":"Austral Biologicals","version":"1.0","type":"link"}