{"product_id":"tm-101d","title":"Anti-RAP74, IgG Fraction, Monoclonal, Clone 7B3\/E10","description":"\u003cp\u003eMouse monoclonal antibody (clone 7B3\/E10) which recognizes an epitope located at aa411 - aa435 of RAP74. This antibody has been purified using Protein G.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStability:\u003c\/strong\u003e At least 1 year at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological activity:\u003c\/strong\u003e This antibody reacts with RAP-74 in ELISA, immunoprecipitation and Western blots. This antibody can be used for affinity purification of the human RNA Polymerase II transcription complex. IgG Subtype: IgG-1.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Solution at 0.25 mg\/mL in 0.1M Tris-HCl (pH 7.5)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReferences:\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e1. Affinity Purification of a Human RNA polymerase II complex using monoclonal antibodies against transcription factor IIF. Cho, H. et al., J. Biol. Chem. 272(17), 11495-11502, (1997)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- AB_PUBLISHED_USE_START --\u003e\n\u003csection class=\"ab-published-use\"\u003e\n\u003ch3 id=\"ab-published-use-tm-101d\"\u003ePublished product use\u003c\/h3\u003e\n\u003cp\u003eA peer-reviewed study reported use of TM-101D-55 for RAP74\/TFIIF detection:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blot:\u003c\/strong\u003e TM-101D-55 was used as the anti-RAP74\/TFIIF antibody to verify TFIIF in a purified Mediator-RNA polymerase II-TFIIF assembly. \u003ca href=\"https:\/\/doi.org\/10.1371\/journal.pbio.1000603\" rel=\"noopener\" target=\"_blank\"\u003eBernecky et al., PLOS Biology (2011)\u003c\/a\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- AB_PUBLISHED_USE_END --\u003e\n\u003cp class=\"ab-datasheet-link\"\u003e\u003ca href=\"https:\/\/helix-chartreuse-ypja.squarespace.com\/s\/Austral_Biologicals_TM-101D_Datasheet.pdf\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003eDownload technical datasheet (PDF)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Austral Biologicals","offers":[{"title":"150 µg","offer_id":52518884049198,"sku":"TM-101D-55","price":425.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1006\/2846\/3918\/files\/austral-biologicals-tm-101d-150ug.png?v=1788007966","url":"https:\/\/www.australbiologicals.com\/products\/tm-101d","provider":"Austral Biologicals","version":"1.0","type":"link"}