Anti-RAP74, IgG Fraction, Monoclonal, Clone 7B3/E10
150 µg
$425.00
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Anti-RAP74, IgG Fraction, Monoclonal, Clone 7B3/E10
Catalog no.
TM-101D-55
$425.00
Mouse monoclonal antibody (clone 7B3/E10) which recognizes an epitope located at aa411 - aa435 of RAP74. This antibody has been purified using Protein G.
- Storage: Store at -20°C.
- Stability: At least 1 year at -20°C.
- Biological activity: This antibody reacts with RAP-74 in ELISA, immunoprecipitation and Western blots. This antibody can be used for affinity purification of the human RNA Polymerase II transcription complex. IgG Subtype: IgG-1.
- Formulation: Solution at 0.25 mg/mL in 0.1M Tris-HCl (pH 7.5)
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References:
1. Affinity Purification of a Human RNA polymerase II complex using monoclonal antibodies against transcription factor IIF. Cho, H. et al., J. Biol. Chem. 272(17), 11495-11502, (1997)
Published product use
A peer-reviewed study reported use of TM-101D-55 for RAP74/TFIIF detection:
- Western blot: TM-101D-55 was used as the anti-RAP74/TFIIF antibody to verify TFIIF in a purified Mediator-RNA polymerase II-TFIIF assembly. Bernecky et al., PLOS Biology (2011).