{"product_id":"pt-5025a","title":"Rabbit anti-Human SHPRH IgG fraction polyclonal antibody","description":"\u003cp\u003eIgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (DKTKKQAVGSPRKIQKETRK) corresponding to amino acids 521-540 of human SHPRH. The IgG fraction was purified using Protein G-Sepharose.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStability:\u003c\/strong\u003e Stable at least one year at -20°C. Avoid repeated freezing and thawing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological activity:\u003c\/strong\u003e This polyclonal antibody reacts in ELISA and Western blot (1:500 dilution) using the immunizing peptide. SHPRH is a member of the SWI\/SNF family of ATPaes\/helicases, it posesses a C3HC4 RING motif characteristic of ubiquitin ligase protein and it has a fundamental role in DNA repair mechanism. Upon genotoxic stress, SHPRH associates with PCNA, RAD18, and the ubiquitin-conjugating enzyme UBC(E2) and promotes methyl methanesulfonate (MMS)-induced PCNA polyubiquitation suppressing genomic instability. SHPRH gene is mutated in a number of cancer cell lines, including those from melanomas and ovarian cancer.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Solution at 1 mg\/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReferences:\u003c\/strong\u003e Sood, R. et al. (2003) Genomics 82, 153-161. Unk, I. et al. (2006) Proc. Natl. Acad. Sci 103, 18107-18112. Motegi, A. et al. (2006) J. Cell Biol. 175, 703-708.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp class=\"ab-datasheet-link\"\u003e\u003ca href=\"https:\/\/helix-chartreuse-ypja.squarespace.com\/s\/Austral_Biologicals_PT-5025A_Datasheet.pdf\" target=\"_blank\" rel=\"noopener\"\u003e\u003cstrong\u003eDownload technical datasheet (PDF)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Austral Biologicals","offers":[{"title":"1mg","offer_id":52517556125998,"sku":"PT-5025a-9","price":425.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1006\/2846\/3918\/files\/austral-biologicals-pt-5025a-1mg.png?v=1788008100","url":"https:\/\/www.australbiologicals.com\/products\/pt-5025a","provider":"Austral Biologicals","version":"1.0","type":"link"}