{"product_id":"pt-5020a","title":"Rabbit anti-Human Poly[ADP-ribose] Polymerase-1 (PARP) IgG fraction polyclonal antibody","description":"\u003cp\u003eIgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (GVDEVAKKKSKK) corresponding to amino acids 214-225 of human PARP. The IgG fraction was purified using Protein G-Sepharose.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStability:\u003c\/strong\u003e Stable at least one year at -20°C. Avoid repeated freezing and thawing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological activity:\u003c\/strong\u003e This polyclonal antibody can be used in immunocytochemistry (1:100 dilution) and it recognizes PARP (115 kDa) by Western blot assays (1: 1000 dilution). Poly [ADP-ribose] polymerase-1 (PARP) is a nuclear protein that catalyses the poly ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. It is activated by DNA strand breaks and plays a role in DNA-base excision repair, DNA-damage signalling, transcription regulation and proteosomal function. PARP is cleaved by caspases during apoptosis to yield two apoptotic fragments, the amino-terminal DNA binding domain (25 kDa) and the carboxy-terminal catalytic domain (85 kDa). PARP cleavage facilitates the cellular disassembly and serves as a marker of cell undergoing apoptosis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Solution at 1 mg\/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReferences:\u003c\/strong\u003e Cherney, B.W. et al. (1987) Proc. Natl. Acad. Sci. USA 84, 8370-8374. Olivier, F.J. et al. (1999) Am. J. Hum. Genet. 64, 1282-1288. Kim, M.Y. et al. (2005) Genes \u0026amp; Dev. 19, 1951-1967. Schreiber, V. et al. (2006) Nat. Rev. Mol. Cell Biol. 7, 517-528.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp class=\"ab-datasheet-link\"\u003e\u003ca href=\"https:\/\/helix-chartreuse-ypja.squarespace.com\/s\/Austral_Biologicals_PT-5020A_Datasheet.pdf\" target=\"_blank\" rel=\"noopener\"\u003e\u003cstrong\u003eDownload technical datasheet (PDF)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Austral Biologicals","offers":[{"title":"1mg","offer_id":52517556093230,"sku":"PT-5020a-9","price":425.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1006\/2846\/3918\/files\/austral-biologicals-pt-5020a-1mg.png?v=1788007966","url":"https:\/\/www.australbiologicals.com\/products\/pt-5020a","provider":"Austral Biologicals","version":"1.0","type":"link"}