Rabbit anti-Human Poly[ADP-ribose] Polymerase-1 (PARP) IgG fraction polyclonal antibody

IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (GVDEVAKKKSKK) corresponding to amino acids 214-225 of human PARP. The IgG fraction was purified using Protein G-Sepharose.

• Storage: Store at -20°C.
• Stability: Stable at least one year at -20°C. Avoid repeated freezing and thawing.
• Biological activity: This polyclonal antibody can be used in immunocytochemistry (1:100 dilution) and it recognizes PARP (115 kDa) by Western blot assays (1: 1000 dilution). Poly [ADP-ribose] polymerase-1 (PARP) is a nuclear protein that catalyses the poly ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. It is activated by DNA strand breaks and plays a role in DNA-base excision repair, DNA-damage signalling, transcription regulation and proteosomal function. PARP is cleaved by caspases during apoptosis to yield two apoptotic fragments, the amino-terminal DNA binding domain (25 kDa) and the carboxy-terminal catalytic domain (85 kDa). PARP cleavage facilitates the cellular disassembly and serves as a marker of cell undergoing apoptosis.
• Formulation: Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
• References: Cherney, B.W. et al. (1987) Proc. Natl. Acad. Sci. USA 84, 8370-8374. Olivier, F.J. et al. (1999) Am. J. Hum. Genet. 64, 1282-1288. Kim, M.Y. et al. (2005) Genes & Dev. 19, 1951-1967. Schreiber, V. et al. (2006) Nat. Rev. Mol. Cell Biol. 7, 517-528.

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