{"product_id":"pt-5001a","title":"Rabbit anti-Human N-Myc IgG fraction polyclonal antibody","description":"\u003cp\u003eIgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (SPYVESEDAPPQKK) corresponding to amino acids 337-349 of human N-Myc. The IgG fraction was purified using Protein G-Sepharose.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStability:\u003c\/strong\u003e Stable at least one year at -20°C. Avoid repeated freezing and thawing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological activity:\u003c\/strong\u003e This polyclonal antibody reacts in ELISA and Western blot assays (1: 500 dilution) using the immunizing peptide. N-Myc is a 49.5 kDa nuclear phosphoprotein that contains a basic helix-loop-helix leucine zipper domain involved in DNA binding and protein dimerization. It regulates gene expression by dimerizing with itself or other helix-loop-helix proteins and this complex binds to DNA E-box sequences of regulated genes. N-Myc is expressed in high levels during embryonic development and in the adult during B-cell differentiation. It is overexpressed in a variety of tumors, aminly neuroblastomas and is associated with rapid progression and poor prognosis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Solution at 1 mg\/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReferences:\u003c\/strong\u003e Slamon, D.J. et al. (1986) Science 232, 768-772. Eisenman, R.N. (2001) Genes \u0026amp; Dev. 15, 2023-2030. Knoepfler, P.S. et al. (2006) EMBO J. 25, 2723-2734.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp class=\"ab-datasheet-link\"\u003e\u003ca href=\"https:\/\/helix-chartreuse-ypja.squarespace.com\/s\/Austral_Biologicals_PT-5001A_Datasheet.pdf\" target=\"_blank\" rel=\"noopener\"\u003e\u003cstrong\u003eDownload technical datasheet (PDF)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Austral Biologicals","offers":[{"title":"1mg","offer_id":52517555929390,"sku":"PT-5001a-9","price":425.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1006\/2846\/3918\/files\/austral-biologicals-pt-5001a-1mg.png?v=1788008060","url":"https:\/\/www.australbiologicals.com\/products\/pt-5001a","provider":"Austral Biologicals","version":"1.0","type":"link"}