Rabbit anti-Human PARK 7 (DJ-1) IgG fraction polyclonal antibody

IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (AAQVKAPLVLKD) corresponding to amino acids 178-189 of human PARK 7. The IgG fraction was purified using Protein G-Sepharose.

• Storage: Store at -20°C.
• Stability: Stable at least one year at -20°C. Avoid repeated freezing and thawing.
• Biological activity: This polyclonal antibody can be used in ELISA (1:1,000 dilution), immunocytochemistry (1:100 dilution) and it recognizes PARK 7 (20 kDa) in Western blot of HeLa lysate(1:1,000 dilution). PARK 7, also known as DJ-1 is a highly conserved cytoplasmatic protein and acts as a positive regulator of androgen receptor-dependent transcription. The expression of PARK 7 is induced by growth signals, and it is translocated from the cytoplasm to nucleus during S-phase of the cell cycle. PARK 7 also may function as redox-sensitive chaperone and as sensor for oxidative stress. Mutations in PARK 7 are associated with the autosomal recessive early-onset Parkinson's disease.
• Formulation: Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
• References: Nagakubo, D. et al. (1997) Biochem. Biophys. Res. Commun. 231, 509-513. Takahashi, K. et al. (2001) J. Biol. Chem. 276, 37556-37563. Miller, D.W. et al. (2003) J. Biol. Chem. 278, 36588-36595. Cookson, M.R. (2005) Annu. Rev. Biochem. 74, 29-52. Moore, D.J. (2006) Biochem. Soc. Trans. 34, 749-753.

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