{"product_id":"pca-2115a","title":"Rabbit anti-Human NFkappaB p65 IgG fraction polyclonal antibody","description":"\u003cp\u003eIgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (DMDFSALLSQISS) corresponding to amino acids 539-551 of human NFkappaB p65. The IgG fraction was purified using Protein G-Sepharose.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStability:\u003c\/strong\u003e Stable at least one year at -20°C. Avoid repeated freezing and thawing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological activity:\u003c\/strong\u003e This polyclonal antibody reacts in ELISA (1: 50 dilution) and Western blot (1: 1,000) using the immunizing peptide. Nuclear factor kappa light chain enhancer in B cells (NFkappaB) is a transcription regulator that consists of two subunits, 50 kDa DNA binding subunit (p50) and 65 kDa transactivation subunit (p65\/Rel1A). The p50\/p65 heterodimer predominates in many cells types. p65 shows a weak DNA-binding site which could contribute directly to DNA binding of the NFkappaB complex. The inhibitory effect of IkappaB upon NFkappaB in the cytoplasm is exerted primarily through the interaction with p65 subunit.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Solution at 1 mg\/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReferences:\u003c\/strong\u003e Ruben, S.M. et al. (1999) Mol. Cell. Biol. 12, 444-454. Saccani, S. et al. (2004) J. Exp. Med. 200, 107-113. Vermeulen, L. et al. (2006) Cancer Treat. Res. 130, 89-102.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp class=\"ab-datasheet-link\"\u003e\u003ca href=\"https:\/\/helix-chartreuse-ypja.squarespace.com\/s\/Austral_Biologicals_PCA-2115A_Datasheet.pdf\" target=\"_blank\" rel=\"noopener\"\u003e\u003cstrong\u003eDownload technical datasheet (PDF)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Austral Biologicals","offers":[{"title":"1mg","offer_id":52517554684206,"sku":"PCA-2115a-9","price":425.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1006\/2846\/3918\/files\/austral-biologicals-pca-2115a-1mg.png?v=1788008205","url":"https:\/\/www.australbiologicals.com\/products\/pca-2115a","provider":"Austral Biologicals","version":"1.0","type":"link"}