Rabbit anti-Human Cullin3 (CUL 3) IgG fraction polyclonal antibody

IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (MSNLSKGTGSRK) corresponding to amino acids 1-12 of human Cullin 3. The IgG fraction was purified using Protein G-Sepharose.

• Storage: Store at -20°C.
• Stability: Stable at least one year at -20°C. Avoid repeated freezing and thawing.
• Biological activity: This polyclonal antibody reacts in ELISA (1: 100 dilution) and Western blot (1: 1,000) using the immunizing peptide. Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 3 (CUL 3) is involved in the ubiquitination of cyclin E forming a tight complex with this protein which leads to its degradation by proteosomes.
• Formulation: Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
• References: Du, M. et al. (1998) J. Biol. Chem. 273, 24289-24292. Singer, J.D. et al. (1999) Genes & Dev. 13, 2375-2387. Van den Heuvel, S. (2004) Curr. Biol. 14, R59-61. Sumara, I. et al. (2007) Dev. Cell. 12, 887-900.

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