Rabbit anti-Human Cullin2 (CUL 2) IgG fraction polyclonal antibody

IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (RSQASADEYSYVA) corresponding to amino acids 733-745 of human Cullin 2. The IgG fraction was purified using Protein G-Sepharose.

• Storage: Store at -20°C.
• Stability: Stable at least one year at -20°C. Avoid repeated freezing and thawing.
• Biological activity: This polyclonal antibody reacts in ELISA (1: 100 dilution) and Western blot (1: 1,000) using the immunizing peptide. Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 2 (CUL 2) is a component of E3 ubiquitin ligase complexes which controls the proteolysis of G1 cell cycle regulatory proteins. It forms a complex with an inactive transcriptional elongation complex formed by three subunits elongain C, elongain B and VHL.
• Formulation: Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
• References: Michel, J.J. & Xiong, Y. (1998) Cell Growth Differ. 9, 435-449. Liakopoulos, D. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 5510-5515.

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