Human recombinant MIP-1 alpha

Macrophage Inflammatory Protein-1( (MIP-1 alpha) is part of the CC subfamily of the chemokine superfamily of chemoattractant cytokines. MIP-1 alpha affects the inflammatory response of blood monocytes, tissue macrophages, and T cells. MIP-1 alpha also enhances the formation of granulocyte-macrophage colonies stimulated by GM-CSF or M-CSF. Human MIP-1 alpha is composed of 69 amino acid residues with not possible N-linked glycosylation sites, and several possible O-linked sites. Human MIP-1 alpha is 60% homologous with human MIP-1 beta, 75% homologous with murine MIP-1 alpha, and has been shown to be active on murine cells. Human recombinant MIP-1 alpha is produced in genetically engineered yeast, and purified by sequential chromatography.

• Molecular weight: 8 kDa
• Purity: Over 95% pure by SDS gel electrophoresis, with low level of endotoxin.
• Storage: Store at -80°C.
• Stability: Several months at -80°C.
• Biological activity: The biological activity of recombinant human MIP-1 alpha is determined by its use as a cofactor for CSF-dependent myelopoiesis.
• Formulation: In water at a concentration of 1.0 mg protein/ml
• References: 1Nathan, C.F. (1987)J. Clin. Invest. 79:319
  2. Wolpe S.D. et al. (1989) Proc. Natl. Acad. Sci. (USA) 86:612
  3. Tekamp-Olson. P. et al. (1990) J. Exp. Med. 172:911
  2. Dunlop, et al (1992) Blood 79: 2221
  3. Suzuki, K. et al. (1989) J. Exp. Med. 169:1895

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