Anti-Glycoprotein 1 of Seoul Virus (SEOV), IgG Fraction, Monoclonal, Clone 2C8/B11

Mouse monoclonal antibody IgG fraction (clone 2C8/B11) obtained by immunizing mice with purified recombinant SEOV Glycoprotein 1 (G1). The IgG fraction was purified using Protein G-Sepharose.

• Storage: Store at -20°C.
• Stability: Stable at least one year at -20°C. Avoid repeated freezing and thawing.
• Biological activity: This antibody can be used in ELISA (1:10,000 dilution) and Western blot (1:20,000 dilution) analysis. It recognizes specifically G1 glycoprotein of SEOV. Hantaviruses (family Bunyaviridae) are enveloped viruses that contain a tripartite, negative-stranded RNA genome encoding an RNA-dependent RNA polymerase (L), two integral membrane glycoproteins (G1 and G2), and a nucleocapsid (N) protein. The G1 and G2 glycoproteins form heterodimers and they attach the virion to a cell receptor and to promote fusion membrane after endocytosis of the virion. G1 can dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis. SEOV is a hantavirus causing a mild to moderate form of hemorrhagic fever with renal syndrome that is distributed mainly in Asia and Europe. It is transmitted by the rodents Rattus rattus and Rattus norvegicus.
• Formulation: Solution at 1.0 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
• References:
  
  1. Zhang, Y-Z et al. (2009) Emerg. Infect. Dis. 15, 200-206.
  2. Lokugamage, N. et al. (2004) Microbiol. Immunol. 48, 843-851.
  3. Kim, Y.S. et al. (1995) Nephon 71, 419-427.
  4. Antic, D. et al. (1991) Virus Res. 19, 47-58.
  5. Arikawa, J. et al. (1990) Virology 176, 114-125.

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