Tumor Necrosis Factor (TNF alpha), Human Recombinant

Human Tumor necrosis factor (TNF- alpha), also known as Cachectin, is a cytokine composed of 157 amino acid residues, having a molecular weight of 17 kDa (monomer). There is 79% homology between human and murine TNF- alpha, including conserved cysteine residues which form an intrachain disulfide bridge. Biological effects of this cytokine include antitumor activity in vivo and in vitro, activation of polymorphonuclear leukocytes, antiviral activity, and the induction of the release if Interleukin-1 or colony stimulating factors from a variety of sources. Human TNF- alpha (Cachectin) is produced in genetically engineered yeast, and purified by sequential chromatography.

• Molecular weight: 17 kDa
• Purity: Over 95% pure by SDS gel electrophoresis.
• Storage: Store at -80°C.
• Stability: At least 1 year at -80°C.
• Biological activity: The cell cytolytic activity is determined in the L929 cells (3 x 104 cells/assay) and it is 2-4 x 108 U/mg.
• Formulation: At concentration of 1 mg/mL in 0.02M Tris-HCl/ 0.15 M NaCl (pH 8.0)
• References:
  
  1. Feinstein, R., Kanety, H., Papa, M.Z., lunenfeld, B., and Karasik, A., (1993) The Journal of Biological Chemistry, Vol 268, No. 35, December, 26055-26058
  2. Kalthoff, H., Roeder, C., Brockhaus, M., Thiele, H.G., and Schmiegel, W., (1993) The Journal of Biological Chemistry, Vol 268, No. 4, February, 2762-2766 3. Chaturvedi, M.M. et al. (1994). J. Biol Chem. 269, 14575-14583.

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