{"product_id":"gf-080","title":"Platelet derived Growth Factor AA (PDGF-AA), Human Recombinant","description":"\u003cp\u003eHuman PDGF-AA homodimer is produced by genetically engineered yeast, and purified by ionic exchange chromatography and reverse-phase HPLC. This polypeptide is composed of two identical polypeptide chains attached by disulfide bonds.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e Over 95% pure by N-terminal amino acid sequencing, amino acid composition, HPLC analysis, and SDS gel electrophoresis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at +4°C. Reconstitution is best in water or buffer near neutral pH.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStability:\u003c\/strong\u003e Several months at +4°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological activity:\u003c\/strong\u003e Thematerial is fully active as measured by the mitogenic assay involving the stimulation of 3H-thymidine incorporation into NIH-3T3 cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Lyophilized powder\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReferences:\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e1. Heldin, C.H. and Westmark, B. (1990) Celll Regul. 1: 555-566\u003cbr\u003e2. Raines, E. W. et al. (1990) Handbook of Experimental Pharmacology (Sporn, M.B. and Roberts, A.B. eds) Vol 9i5: pages 173-262\u003cbr\u003e3. Ostman, A. et al. (1989) Growth Factors 1: 219-229\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- AB_PUBLISHED_USE_START --\u003e\n\u003csection class=\"ab-published-use ab-published-use-complete\"\u003e\n\u003ch3 id=\"ab-published-use-gf-080\"\u003ePublications using Austral Biologicals GF-080\u003c\/h3\u003e\n\u003cp\u003eThe following scientific publications report use of PDGF-AA from Austral Biologicals:\u003c\/p\u003e\n\u003col class=\"ab-publication-list ab-publication-list-complete\"\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1158\/1078-0432.CCR-17-1258\" rel=\"noopener\" target=\"_blank\"\u003eOlaratumab Exerts Antitumor Activity in Preclinical Models of Pediatric Bone and Soft Tissue Tumors through Inhibition of Platelet-Derived Growth Factor Receptor α\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eLowery et al., Clinical Cancer Research 24 847-857 (2018).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1016\/j.jim.2013.11.018\" rel=\"noopener\" target=\"_blank\"\u003eIdentification and elimination of target-related matrix interference in a neutralizing anti-drug antibody assay\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eSchwickart et al., Journal of Immunological Methods 403 52-61 (2014).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1007\/s10637-012-9912-9\" rel=\"noopener\" target=\"_blank\"\u003eLY2801653 is an orally bioavailable multi-kinase inhibitor with potent activity against MET, MST1R, and other oncoproteins, and displays anti-tumor activities in mouse xenograft models\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eYan et al., Investigational New Drugs 31 833-844 (2013).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1158\/1535-7163.MCT-04-0114\" rel=\"noopener\" target=\"_blank\"\u003eTargeting the platelet-derived growth factor receptor α with a neutralizing human monoclonal antibody inhibits the growth of tumor xenografts: Implications as a potential therapeutic target\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eLoizos et al., Molecular Cancer Therapeutics 4 369-379 (2005).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1038\/sj.bjp.0703771\" rel=\"noopener\" target=\"_blank\"\u003ePlatelet‐derived growth factor causes endothelium‐independent relaxation of rabbit mesenteric artery via the release of a prostanoid\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eYamawaki et al., British Journal of Pharmacology 131 1546-1552 (2000).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1177\/00220345980770100601\" rel=\"noopener\" target=\"_blank\"\u003ePDGF-α Receptor Subunit Expression Down-regulated by IL-1β in Human Periodontal Ligament Cells\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eOates et al., Journal of Dental Research 77 1791-1798 (1998).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1038\/bjc.1996.496\" rel=\"noopener\" target=\"_blank\"\u003eDifferential responses of scirrhous and well-differentiated gastric cancer cells to orthotopic fibroblasts\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eYashiro et al., British Journal of Cancer 74 1096-1103 (1996).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1002\/(SICI)1097-4652(199601)166:1%3C188::AID-JCP20%3E3.0.CO;2-A\" rel=\"noopener\" target=\"_blank\"\u003ePro-inflammatory cytokines downregulate platelet derived growth factor-α receptor gene expression in human osteoblastic cells\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eKose et al., Journal of Cellular Physiology 166 188-197 (1996).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1523\/JNEUROSCI.15-11-07095.1995\" rel=\"noopener\" target=\"_blank\"\u003ePDGFs protect hippocampal neurons against energy deprivation and oxidative injury: evidence for induction of antioxidant pathways\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eCheng et al., The Journal of Neuroscience 15 7095-7104 (1995).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1042\/bj3100585\" rel=\"noopener\" target=\"_blank\"\u003ePlatelet-derived growth factors-AA and -BB regulate collagen and collagenase gene expression differentially in human fibroblasts\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eTan et al., Biochemical Journal 310 585-588 (1995).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1002\/jcp.1041620308\" rel=\"noopener\" target=\"_blank\"\u003eReceptor binding of PDGF‐AA and PDGF‐BB, and the modulation of PDGF receptors by TGF‐β, in human periodontal ligament cells\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eOates et al., Journal of Cellular Physiology 162 359-366 (1995).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1002\/jbmr.5650100114\" rel=\"noopener\" target=\"_blank\"\u003eInterleukin-1 modulates phosphorylation of proteins in human osteoblastic cells\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eKang et al., Journal of Bone and Mineral Research 10 96-105 (1995).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/section\u003e\n\u003c!-- AB_PUBLISHED_USE_END --\u003e\n\u003cp class=\"ab-datasheet-link\"\u003e\u003ca href=\"https:\/\/helix-chartreuse-ypja.squarespace.com\/s\/Austral_Biologicals_GF-080_Datasheet.pdf\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003eDownload technical datasheet (PDF)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Austral Biologicals","offers":[{"title":"10 µg","offer_id":52518874546478,"sku":"GF-080-3","price":150.0,"currency_code":"USD","in_stock":true},{"title":"100 µg","offer_id":52518874579246,"sku":"GF-080-5","price":850.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1006\/2846\/3918\/files\/austral-biologicals-gf-080-10ug.png?v=1788008648","url":"https:\/\/www.australbiologicals.com\/products\/gf-080","provider":"Austral Biologicals","version":"1.0","type":"link"}