{"product_id":"gf-070","title":"Platelet-derived Growth Factor BB (PDGF-BB), Human Recombinant","description":"\u003cp\u003eHuman PDGF-BB homodimer is produced by genetically engineered yeast, and purified by sequential chromatography and ionic exchange HPLC. This polypeptide is composed of two identical polypeptide chains attached by disulfide bonds. As it has been described in the natural product, PDGF-BB is partially clipped at Arg32-Thr33. This cleavage is visible upon reduction of the polypeptide with a reducing agent. Note: this clipping does not affect the biological activity.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e Over 97% pure by N-terminal amino acid sequencing, amino acid composition, HPLC analysis, and SDS gel electrophoresis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at +4°C. Reconstitution is best in water or buffer near neutral pH.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStability:\u003c\/strong\u003e Several months at +4°C. In solution, at pH 4-7, is stable for 2-3 months at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological activity:\u003c\/strong\u003e The material is fully active as determined by its mitogenic activity measured by stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Lyophilized powder\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReferences:\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e1. Ross, R. et al. (1986) Cell 46:155\u003cbr\u003e2. Johnson, A. et al. (1984) EMBO J.3:921\u003cbr\u003e3. Waterfield, M.D. et al. (1983) Nature 303: 35\u003cbr\u003e4. Brake, A.J. et al. (1984) Proc. Natl. Acad. Sci. U.S.A. 81(15):4642-4646. doi:10.1073\/pnas.81.15.4642\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- AB_PUBLISHED_USE_START --\u003e\n\u003csection class=\"ab-published-use ab-published-use-complete\"\u003e\n\u003ch3 id=\"ab-published-use-gf-070\"\u003ePublications using Austral Biologicals GF-070\u003c\/h3\u003e\n\u003cp\u003eThe following scientific publications report use of PDGF-BB from Austral Biologicals:\u003c\/p\u003e\n\u003col class=\"ab-publication-list ab-publication-list-complete\"\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1002\/1873-3468.14345\" rel=\"noopener\" target=\"_blank\"\u003eBasic fibroblast growth factor uniquely stimulates quiescent vascular smooth muscle cells and induces proliferation and dedifferentiation\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eTsuji‐Tamura et al., FEBS Letters 596 1686-1699 (2022).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1158\/1078-0432.CCR-17-1258\" rel=\"noopener\" target=\"_blank\"\u003eOlaratumab Exerts Antitumor Activity in Preclinical Models of Pediatric Bone and Soft Tissue Tumors through Inhibition of Platelet-Derived Growth Factor Receptor α\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eLowery et al., Clinical Cancer Research 24 847-857 (2018).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1167\/iovs.14-13945\" rel=\"noopener\" target=\"_blank\"\u003ePericyte Chemomechanics and the Angiogenic Switch: Insights Into the Pathogenesis of Proliferative Diabetic Retinopathy?\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eDurham et al., Investigative Ophthalmology \u0026amp; Visual Science 56(6):3441-3459 (2015).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1074\/jbc.M113.475285\" rel=\"noopener\" target=\"_blank\"\u003eDissecting the Roles of Tyrosines 490 and 785 of TrkA Protein in the Induction of Downstream Protein Phosphorylation Using Chimeric Receptors\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eBiarc et al., Journal of Biological Chemistry 288 16606-16618 (2013).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1074\/mcp.M111.013375\" rel=\"noopener\" target=\"_blank\"\u003eThe Induction of Serine\/Threonine Protein Phosphorylations by a PDGFR\/TrkA Chimera in Stably Transfected PC12 Cells\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eBiarc et al., Molecular \u0026amp; Cellular Proteomics 11 15-30 (2012).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1152\/ajpcell.00536.2009\" rel=\"noopener\" target=\"_blank\"\u003eProtein tyrosine phosphatase PTPεM negatively regulates PDGF β-receptor signaling induced by high glucose and PDGF in vascular smooth muscle cells\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eShimizu et al., American Journal of Physiology-Cell Physiology 299 C1144-C1152 (2010).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1152\/ajpcell.00206.2009\" rel=\"noopener\" target=\"_blank\"\u003eROS and PDFG-β receptors are critically involved in indoxyl sulfate actions that promote vascular smooth muscle cell proliferation and migration\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eShimizu et al., American Journal of Physiology-Cell Physiology 297 C389-C396 (2009).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1091\/mbc.E05-03-0263\" rel=\"noopener\" target=\"_blank\"\u003eDivergent Roles of c-Src in Controlling Platelet-derived Growth Factor-dependent Signaling in Fibroblasts\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eShah et al., Molecular Biology of the Cell 16 5418-5432 (2005).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1002\/hep.20719\" rel=\"noopener\" target=\"_blank\"\u003eNAD(P)H oxidase plays a crucial role in PDGF‐induced proliferation of hepatic stellate cells\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eAdachi et al., Hepatology 41 1272-1281 (2005).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1093\/hmg\/ddi161\" rel=\"noopener\" target=\"_blank\"\u003eSustained ERK1\/2 but not STAT1 or 3 activation is required for thanatophoric dysplasia phenotypes in PC12 cells\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eNowroozi et al., Human Molecular Genetics 14 1529-1538 (2005).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1128\/MCB.25.7.2593-2606.2005\" rel=\"noopener\" target=\"_blank\"\u003eRole of Phosphoinositide 3-Kinase Regulatory Isoforms in Development and Actin Rearrangement\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eBrachmann et al., Molecular and Cellular Biology 25 2593-2606 (2005).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1158\/1535-7163.MCT-04-0114\" rel=\"noopener\" target=\"_blank\"\u003eTargeting the platelet-derived growth factor receptor α with a neutralizing human monoclonal antibody inhibits the growth of tumor xenografts: Implications as a potential therapeutic target\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eLoizos et al., Molecular Cancer Therapeutics 4 369-379 (2005).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1271\/bbb.68.1817\" rel=\"noopener\" target=\"_blank\"\u003eEpigallocatechin-3-O-gallate Inhibits Fibroblast Contraction of Floating Collagen Gel: Interaction between Epigallocatechin-3-O-gallate and Platelet Derived Growth Factor\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eSuzuki et al., Bioscience, Biotechnology, and Biochemistry 68 1817-1820 (2004).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.3109\/13506120209114099\" rel=\"noopener\" target=\"_blank\"\u003eAcute-phase, but not constitutive serum amyloid A (SAA) is chemotactic for cultured human aortic smooth muscle cells\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eKumon et al., Amyloid 9 237-241 (2002).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1093\/ps\/81.8.1191\" rel=\"noopener\" target=\"_blank\"\u003eThe effect of hepatocyte growth factor on turkey satellite cell proliferation and differentiation\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eZeng et al., Poultry Science 81 1191-1198 (2002).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1038\/sj.bjp.0703771\" rel=\"noopener\" target=\"_blank\"\u003ePlatelet‐derived growth factor causes endothelium‐independent relaxation of rabbit mesenteric artery via the release of a prostanoid\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eYamawaki et al., British Journal of Pharmacology 131 1546-1552 (2000).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1152\/ajpendo.2000.279.3.E570\" rel=\"noopener\" target=\"_blank\"\u003eTranscriptional regulation of connective tissue growth factor by cortisol in osteoblasts\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003ePereira et al., American Journal of Physiology-Endocrinology and Metabolism 279 E570-E576 (2000).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1152\/ajpheart.1999.277.1.H318\" rel=\"noopener\" target=\"_blank\"\u003eImpairment of EDR by a long-term PDGF treatment in organ-cultured rabbit mesenteric artery\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eYamawaki et al., American Journal of Physiology-Heart and Circulatory Physiology 277 H318-H323 (1999).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1074\/jbc.274.27.19246\" rel=\"noopener\" target=\"_blank\"\u003eTransient Effect of Platelet-derived Growth Factor on GLUT4 Translocation in 3T3-L1 Adipocytes\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eWang et al., Journal of Biological Chemistry 274 19246-19253 (1999).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1073\/pnas.96.13.7178\" rel=\"noopener\" target=\"_blank\"\u003eComparison of the intracellular signaling responses by three chimeric fibroblast growth factor receptors in PC12 cells\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eRaffioni et al., Proceedings of the National Academy of Sciences 96 7178-7183 (1999).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1074\/jbc.274.10.6783\" rel=\"noopener\" target=\"_blank\"\u003ePlatelet-derived Growth Factor Induces Interleukin-6 Transcription in Osteoblasts through the Activator Protein-1 Complex and Activating Transcription Factor-2\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eFranchimont et al., Journal of Biological Chemistry 274 6783-6789 (1999).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1074\/jbc.273.52.35250\" rel=\"noopener\" target=\"_blank\"\u003eEffect of Transmembrane and Kinase Domain Mutations on Fibroblast Growth Factor Receptor 3 Chimera Signaling in PC12 Cells\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eRaffioni et al., Journal of Biological Chemistry 273 35250-35259 (1998).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1074\/jbc.273.52.35161\" rel=\"noopener\" target=\"_blank\"\u003eMolecular Mechanism of Basic Calcium Phosphate Crystal-induced Activation of Human Fibroblasts\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eMcCarthy et al., Journal of Biological Chemistry 273 35161-35169 (1998).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1177\/00220345980770100601\" rel=\"noopener\" target=\"_blank\"\u003ePDGF-α Receptor Subunit Expression Down-regulated by IL-1β in Human Periodontal Ligament Cells\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eOates et al., Journal of Dental Research 77 1791-1798 (1998).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1152\/ajpcell.1998.274.2.C472\" rel=\"noopener\" target=\"_blank\"\u003eEts-1 is an early response gene activated by ET-1 and PDGF-BB in vascular smooth muscle cells\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eNaito et al., American Journal of Physiology-Cell Physiology 274 C472-C480 (1998).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1128\/MCB.17.7.4169\" rel=\"noopener\" target=\"_blank\"\u003eChimeras of the Native Form or Achondroplasia Mutant (G375C) of Human Fibroblast Growth Factor Receptor 3 Induce Ligand-Dependent Differentiation of PC12 Cells\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eThompson et al., Molecular and Cellular Biology 17 4169-4177 (1997).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1523\/JNEUROSCI.15-11-07095.1995\" rel=\"noopener\" target=\"_blank\"\u003ePDGFs protect hippocampal neurons against energy deprivation and oxidative injury: evidence for induction of antioxidant pathways\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eCheng et al., The Journal of Neuroscience 15 7095-7104 (1995).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1042\/bj3100585\" rel=\"noopener\" target=\"_blank\"\u003ePlatelet-derived growth factors-AA and -BB regulate collagen and collagenase gene expression differentially in human fibroblasts\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eTan et al., Biochemical Journal 310 585-588 (1995).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1172\/JCI117666\" rel=\"noopener\" target=\"_blank\"\u003eFibroblast growth factor stimulates angiotensin converting enzyme expression in vascular smooth muscle cells. Possible mediator of the response to vascular injury\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eFishel et al., Journal of Clinical Investigation 95 377-387 (1995).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/section\u003e\n\u003c!-- AB_PUBLISHED_USE_END --\u003e\n\u003cp class=\"ab-datasheet-link\"\u003e\u003ca href=\"https:\/\/helix-chartreuse-ypja.squarespace.com\/s\/Austral_Biologicals_GF-070_Datasheet.pdf\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003eDownload technical datasheet (PDF)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Austral Biologicals","offers":[{"title":"10 µg","offer_id":52518874448174,"sku":"GF-070-3","price":80.0,"currency_code":"USD","in_stock":true},{"title":"100 µg","offer_id":52518874480942,"sku":"GF-070-5","price":400.0,"currency_code":"USD","in_stock":true},{"title":"500 µg","offer_id":52518874513710,"sku":"GF-070-8","price":1200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1006\/2846\/3918\/files\/austral-biologicals-gf-070-10ug.png?v=1788008656","url":"https:\/\/www.australbiologicals.com\/products\/gf-070","provider":"Austral Biologicals","version":"1.0","type":"link"}