Austral Biologicals GF-070 - Platelet-derived Growth Factor (PDGF-BB) human (RECOMBINANT), 10 µg

Platelet-derived Growth Factor BB (PDGF-BB), Human Recombinant

10 µg
$80.00
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Austral Biologicals GF-070 - Platelet-derived Growth Factor (PDGF-BB) human (RECOMBINANT), 10 µg
  • Austral Biologicals GF-070 - Platelet-derived Growth Factor (PDGF-BB) human (RECOMBINANT), 10 µg
  • Austral Biologicals GF-070 - Platelet-derived Growth Factor (PDGF-BB) human (RECOMBINANT), 10 µg
Platelet-derived Growth Factor BB (PDGF-BB), Human Recombinant

Platelet-derived Growth Factor BB (PDGF-BB), Human Recombinant

Catalog no. GF-070-3
$80.00
Size

Human PDGF-BB homodimer is produced by genetically engineered yeast, and purified by sequential chromatography and ionic exchange HPLC. This polypeptide is composed of two identical polypeptide chains attached by disulfide bonds. As it has been described in the natural product, PDGF-BB is partially clipped at Arg32-Thr33. This cleavage is visible upon reduction of the polypeptide with a reducing agent. Note: this clipping does not affect the biological activity.

  • Purity: Over 97% pure by N-terminal amino acid sequencing, amino acid composition, HPLC analysis, and SDS gel electrophoresis.
  • Storage: Store at +4°C. Reconstitution is best in water or buffer near neutral pH.
  • Stability: Several months at +4°C. In solution, at pH 4-7, is stable for 2-3 months at -20°C.
  • Biological activity: The material is fully active as determined by its mitogenic activity measured by stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells.
  • Formulation: Lyophilized powder
  • References:

    1. Ross, R. et al. (1986) Cell 46:155
    2. Johnson, A. et al. (1984) EMBO J.3:921
    3. Waterfield, M.D. et al. (1983) Nature 303: 35
    4. Brake, A.J. et al. (1984) Proc. Natl. Acad. Sci. U.S.A. 81(15):4642-4646. doi:10.1073/pnas.81.15.4642

Publications using Austral Biologicals GF-070

The following scientific publications report use of PDGF-BB from Austral Biologicals:

  1. Basic fibroblast growth factor uniquely stimulates quiescent vascular smooth muscle cells and induces proliferation and dedifferentiation. Tsuji‐Tamura et al., FEBS Letters 596 1686-1699 (2022).
  2. Olaratumab Exerts Antitumor Activity in Preclinical Models of Pediatric Bone and Soft Tissue Tumors through Inhibition of Platelet-Derived Growth Factor Receptor α. Lowery et al., Clinical Cancer Research 24 847-857 (2018).
  3. Pericyte Chemomechanics and the Angiogenic Switch: Insights Into the Pathogenesis of Proliferative Diabetic Retinopathy?. Durham et al., Investigative Ophthalmology & Visual Science 56(6):3441-3459 (2015).
  4. Dissecting the Roles of Tyrosines 490 and 785 of TrkA Protein in the Induction of Downstream Protein Phosphorylation Using Chimeric Receptors. Biarc et al., Journal of Biological Chemistry 288 16606-16618 (2013).
  5. The Induction of Serine/Threonine Protein Phosphorylations by a PDGFR/TrkA Chimera in Stably Transfected PC12 Cells. Biarc et al., Molecular & Cellular Proteomics 11 15-30 (2012).
  6. Protein tyrosine phosphatase PTPεM negatively regulates PDGF β-receptor signaling induced by high glucose and PDGF in vascular smooth muscle cells. Shimizu et al., American Journal of Physiology-Cell Physiology 299 C1144-C1152 (2010).
  7. ROS and PDFG-β receptors are critically involved in indoxyl sulfate actions that promote vascular smooth muscle cell proliferation and migration. Shimizu et al., American Journal of Physiology-Cell Physiology 297 C389-C396 (2009).
  8. Divergent Roles of c-Src in Controlling Platelet-derived Growth Factor-dependent Signaling in Fibroblasts. Shah et al., Molecular Biology of the Cell 16 5418-5432 (2005).
  9. NAD(P)H oxidase plays a crucial role in PDGF‐induced proliferation of hepatic stellate cells. Adachi et al., Hepatology 41 1272-1281 (2005).
  10. Sustained ERK1/2 but not STAT1 or 3 activation is required for thanatophoric dysplasia phenotypes in PC12 cells. Nowroozi et al., Human Molecular Genetics 14 1529-1538 (2005).
  11. Role of Phosphoinositide 3-Kinase Regulatory Isoforms in Development and Actin Rearrangement. Brachmann et al., Molecular and Cellular Biology 25 2593-2606 (2005).
  12. Targeting the platelet-derived growth factor receptor α with a neutralizing human monoclonal antibody inhibits the growth of tumor xenografts: Implications as a potential therapeutic target. Loizos et al., Molecular Cancer Therapeutics 4 369-379 (2005).
  13. Epigallocatechin-3-O-gallate Inhibits Fibroblast Contraction of Floating Collagen Gel: Interaction between Epigallocatechin-3-O-gallate and Platelet Derived Growth Factor. Suzuki et al., Bioscience, Biotechnology, and Biochemistry 68 1817-1820 (2004).
  14. Acute-phase, but not constitutive serum amyloid A (SAA) is chemotactic for cultured human aortic smooth muscle cells. Kumon et al., Amyloid 9 237-241 (2002).
  15. The effect of hepatocyte growth factor on turkey satellite cell proliferation and differentiation. Zeng et al., Poultry Science 81 1191-1198 (2002).
  16. Platelet‐derived growth factor causes endothelium‐independent relaxation of rabbit mesenteric artery via the release of a prostanoid. Yamawaki et al., British Journal of Pharmacology 131 1546-1552 (2000).
  17. Transcriptional regulation of connective tissue growth factor by cortisol in osteoblasts. Pereira et al., American Journal of Physiology-Endocrinology and Metabolism 279 E570-E576 (2000).
  18. Impairment of EDR by a long-term PDGF treatment in organ-cultured rabbit mesenteric artery. Yamawaki et al., American Journal of Physiology-Heart and Circulatory Physiology 277 H318-H323 (1999).
  19. Transient Effect of Platelet-derived Growth Factor on GLUT4 Translocation in 3T3-L1 Adipocytes. Wang et al., Journal of Biological Chemistry 274 19246-19253 (1999).
  20. Comparison of the intracellular signaling responses by three chimeric fibroblast growth factor receptors in PC12 cells. Raffioni et al., Proceedings of the National Academy of Sciences 96 7178-7183 (1999).
  21. Platelet-derived Growth Factor Induces Interleukin-6 Transcription in Osteoblasts through the Activator Protein-1 Complex and Activating Transcription Factor-2. Franchimont et al., Journal of Biological Chemistry 274 6783-6789 (1999).
  22. Effect of Transmembrane and Kinase Domain Mutations on Fibroblast Growth Factor Receptor 3 Chimera Signaling in PC12 Cells. Raffioni et al., Journal of Biological Chemistry 273 35250-35259 (1998).
  23. Molecular Mechanism of Basic Calcium Phosphate Crystal-induced Activation of Human Fibroblasts. McCarthy et al., Journal of Biological Chemistry 273 35161-35169 (1998).
  24. PDGF-α Receptor Subunit Expression Down-regulated by IL-1β in Human Periodontal Ligament Cells. Oates et al., Journal of Dental Research 77 1791-1798 (1998).
  25. Ets-1 is an early response gene activated by ET-1 and PDGF-BB in vascular smooth muscle cells. Naito et al., American Journal of Physiology-Cell Physiology 274 C472-C480 (1998).
  26. Chimeras of the Native Form or Achondroplasia Mutant (G375C) of Human Fibroblast Growth Factor Receptor 3 Induce Ligand-Dependent Differentiation of PC12 Cells. Thompson et al., Molecular and Cellular Biology 17 4169-4177 (1997).
  27. PDGFs protect hippocampal neurons against energy deprivation and oxidative injury: evidence for induction of antioxidant pathways. Cheng et al., The Journal of Neuroscience 15 7095-7104 (1995).
  28. Platelet-derived growth factors-AA and -BB regulate collagen and collagenase gene expression differentially in human fibroblasts. Tan et al., Biochemical Journal 310 585-588 (1995).
  29. Fibroblast growth factor stimulates angiotensin converting enzyme expression in vascular smooth muscle cells. Possible mediator of the response to vascular injury. Fishel et al., Journal of Clinical Investigation 95 377-387 (1995).

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