{"product_id":"gf-040","title":"Acidic Fibroblast Growth Factor (FGF 1), Human Recombinant","description":"\u003cp\u003eAcidic FGF is a mitogen for cells of mesodermal and neuroectodermal origin, including fibroblasts, endothelial cells, astrocytes, neuroblasts, osteoblasts, and smooth muscle cells. This protein is composed of 140 amino acid residues, and has a 55% homology with basic FGF, including two conserved cysteine residues. Human Acidic FGF is produced by genetically engineered yeast, and purified by heparin-Sepharose affinity chromatography.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 15.5 kDa\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e Over 95% pure by HPLC analysis and SDS gel electrophoresis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at -80°C. It is also possible to keep this protein at low pH (2-5) for a few days.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStability:\u003c\/strong\u003e Unstable without a carrier protein like gelatin or bovine serum albumin. It is recommended to add some of these proteins, prior to aliquoting, for longer storage.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological activity:\u003c\/strong\u003e Human acidic FGF is mitogenic for a variety of normal cells in culture including fibroblasts, glial cells, vascular endothelial cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e 10 mM Tris-HCl, 1.5 M NaCl, pH 7.0 Concentration: 0.50 mg protein\/ml.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReferences:\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e1. Gimenez-Gallego et al., Biochem. Biophys. Res. Comm.(1986) 138:611\u003cbr\u003e2. Thornton et al. (1983), Science 222, 623\u003cbr\u003e3. Gospadorowicz, D. (1987) Methods Enzymol. 147:106\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"ab-datasheet-link\"\u003e\u003ca href=\"https:\/\/helix-chartreuse-ypja.squarespace.com\/s\/Austral_Biologicals_GF-040_Datasheet.pdf\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003eDownload technical datasheet (PDF)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Austral Biologicals","offers":[{"title":"10 µg","offer_id":52518874251566,"sku":"GF-040-3","price":75.0,"currency_code":"USD","in_stock":true},{"title":"50 µg","offer_id":52518874284334,"sku":"GF-040-4","price":300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1006\/2846\/3918\/files\/austral-biologicals-gf-040-10ug.png?v=1788008668","url":"https:\/\/www.australbiologicals.com\/products\/gf-040","provider":"Austral Biologicals","version":"1.0","type":"link"}