{"product_id":"gf-030","title":"Basic Fibroblast Growth Factor (FGF 2), Human Recombinant","description":"\u003cp\u003eHuman Basic Fibroblast Growth Factor is a mitogen that stimulates cell growth for such cells as fibroblasts, endothelial cells, myoblasts, glial cells and smooth muscle cells. Basic FGF is composed of 146 amino acid residues (pro 1 to ser 146), and is 55% homologous with acidic FGF, including two conserved cysteine residues. Human basic FGF is produced by genetically engineered yeast, and purified by heparin-Sepharose affinity chromatography.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight:\u003c\/strong\u003e 17.5 kDa\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e Over 95% pure by N-terminal amino acid sequencing, amino acid composition, HPLC analysis, and SDS gel electrophoresis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at +4°C. Reconstitution is best in water or buffer near neutral pH, since it is unstable in acidic solutions. After reconstitution, storage in aliquots at -80°C is recommended.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStability:\u003c\/strong\u003e Several months, lyophilized or in solution, at pH 6-7 and +4°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological activity:\u003c\/strong\u003e The mitogenic activity of human basic FGF is identical to natural bovine basic FGF, as measured by stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Lyophilized powder\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReferences:\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e1. Gospodarowicz, D. (1975) J. Biol. Chem. 250, 2515-2520.\u003cbr\u003e2. Folkman, J. and Klagsbrun, M. (1987) Science 235,442-447\u003cbr\u003e3. Fox, J., et al. (1996) Journal of Biological Chemistry 271: 12578-12584.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- AB_PUBLISHED_USE_START --\u003e\n\u003csection class=\"ab-published-use ab-published-use-complete\"\u003e\n\u003ch3 id=\"ab-published-use-gf-030\"\u003ePublications using Austral Biologicals GF-030\u003c\/h3\u003e\n\u003cp\u003eThe following scientific publications report use of Austral Biologicals GF-030:\u003c\/p\u003e\n\u003col class=\"ab-publication-list ab-publication-list-complete\"\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1186\/s44330-026-00072-9\" rel=\"noopener\" target=\"_blank\"\u003eCell-cultured PDMS vascular model to allow placement of implant devices\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eOkuno et al., BMC Methods 3:16 (2026).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1063\/5.0222866\" rel=\"noopener\" target=\"_blank\"\u003eGeometrically engineered organoid units and their assembly for pre-construction of organ structures\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eKadotani et al., APL Bioengineering (2024).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1016\/j.biomaterials.2023.122256\" rel=\"noopener\" target=\"_blank\"\u003eHydrolytic hydrogels tune mesenchymal stem cell persistence and immunomodulation for enhanced diabetic cutaneous wound healing\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eMartin et al., Biomaterials (2023).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.3389\/fmedt.2023.1149594\" rel=\"noopener\" target=\"_blank\"\u003eMigration of endothelial cells on the surface of anodized Ni-Ti stent strut\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eWang et al., Frontiers in Medical Technology (2023).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1016\/j.biomaterials.2022.121601\" rel=\"noopener\" target=\"_blank\"\u003eHost type 2 immune response to xenogeneic serum components impairs biomaterial-directed osteo-regenerative therapies\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eMartin et al., Biomaterials (2022).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.3136\/fstr.26.891\" rel=\"noopener\" target=\"_blank\"\u003eAntiangiogenic Activity of Flavonols in Chorioallantoic Membrane (CAM) Assay\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eOkamura et al., Food Science and Technology Research 26:891-896 (2020).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1038\/s42003-020-0881-9\" rel=\"noopener\" target=\"_blank\"\u003eHydrostatic pressure promotes endothelial tube formation through aquaporin 1 and Ras-ERK signaling\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eYoshino et al., Communications Biology (2020).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1083\/jcb.201806065\" rel=\"noopener\" target=\"_blank\"\u003eYAP and TAZ limit cytoskeletal and focal adhesion maturation to enable persistent cell motility\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eMason et al., Journal of Cell Biology (2019).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1016\/j.bbrc.2018.05.182\" rel=\"noopener\" target=\"_blank\"\u003eFluid shear stress suppresses ICAM-1-mediated transendothelial migration of leukocytes in coculture model\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eSakamoto et al., Biochemical and Biophysical Research Communications (2018).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1096\/fj.201700872r\" rel=\"noopener\" target=\"_blank\"\u003eSkeletal cell YAP and TAZ combinatorially promote bone development\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eKegelman et al., FASEB Journal (2018).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1038\/s41598-017-13477-y\" rel=\"noopener\" target=\"_blank\"\u003eA Novel Technique for Accelerated Culture of Murine Mesenchymal Stem Cells that Allows for Sustained Multipotency\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eCaroti et al., Scientific Reports 7:13334 (2017).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1155\/2016\/4651265\" rel=\"noopener\" target=\"_blank\"\u003eProliferation-Related Activity in Endothelial Cells Is Enhanced by Micropower Plasma\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eSuzuki and Yoshino, BioMed Research International (2016).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1007\/s12195-015-0385-8\" rel=\"noopener\" target=\"_blank\"\u003eEndothelial Cell Response Under Hydrostatic Pressure Condition Mimicking Pressure Therapy\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eYoshino et al., Cellular and Molecular Bioengineering 8:296-303 (2015).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.3389\/fendo.2013.00183\" rel=\"noopener\" target=\"_blank\"\u003eBasic fibroblast growth factor predicts cardiovascular disease occurrence in participants from the Veterans Affairs Diabetes Trial\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eZimering et al., Frontiers in Endocrinology 4:183 (2013).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1038\/ncomms2978\" rel=\"noopener\" target=\"_blank\"\u003eHaemodynamically dependent valvulogenesis of zebrafish heart is mediated by flow-dependent expression of miR-21\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eBanjo et al., Nature Communications 4:1978 (2013).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1016\/j.bbrc.2012.06.073\" rel=\"noopener\" target=\"_blank\"\u003eRole of nesprin-1 in nuclear deformation in endothelial cells under static and uniaxial stretching conditions\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eAnno et al., Biochemical and Biophysical Research Communications (2012).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1016\/j.bbrc.2012.01.083\" rel=\"noopener\" target=\"_blank\"\u003eRole of paxillin in the early phase of orientation of vascular endothelial cells exposed to cyclic stretching\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eHuang et al., Biochemical and Biophysical Research Communications (2012).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1093\/ecam\/nep024\" rel=\"noopener\" target=\"_blank\"\u003eBrazilian Propolis Suppresses Angiogenesis by Inducing Apoptosis in Tube-Forming Endothelial Cells through Inactivation of Survival Signal ERK1\/2\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eKunimasa et al., Evidence-Based Complementary and Alternative Medicine (2011).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.2174\/1874120701004010129\" rel=\"noopener\" target=\"_blank\"\u003eCyclic Force Applied to FAs Induces Actin Recruitment Depending on the Dynamic Loading Pattern\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eUeki et al., Open Biomedical Engineering Journal 4:129-134 (2010).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1016\/j.bbrc.2010.06.092\" rel=\"noopener\" target=\"_blank\"\u003eRole of p120-catenin in the morphological changes of endothelial cells exposed to fluid shear stress\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eSakamoto et al., Biochemical and Biophysical Research Communications (2010).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1016\/j.bbrc.2010.04.051\" rel=\"noopener\" target=\"_blank\"\u003eMeasurements of strain on single stress fibers in living endothelial cells induced by fluid shear stress\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eUeki et al., Biochemical and Biophysical Research Communications (2010).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1016\/j.bbrc.2010.04.002\" rel=\"noopener\" target=\"_blank\"\u003eEffect of spatial gradient in fluid shear stress on morphological changes in endothelial cells in response to flow\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eSakamoto et al., Biochemical and Biophysical Research Communications (2010).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1016\/j.bbrc.2010.02.115\" rel=\"noopener\" target=\"_blank\"\u003eDirect measurement of shear strain in adherent vascular endothelial cells exposed to fluid shear stress\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eUeki et al., Biochemical and Biophysical Research Communications (2010).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1016\/S0014-4827(03)00138-1\" rel=\"noopener\" target=\"_blank\"\u003eEx vivo enrichment of mesenchymal cell progenitors by fibroblast growth factor 2\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eBianchi et al., Experimental Cell Research (2003).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1016\/S0304-3835(01)00443-8\" rel=\"noopener\" target=\"_blank\"\u003eResveratrol and quercetin inhibit angiogenesis in vitro\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eIgura et al., Cancer Letters (2001).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1152\/ajpendo.2000.279.3.E570\" rel=\"noopener\" target=\"_blank\"\u003eTranscriptional regulation of connective tissue growth factor by cortisol in osteoblasts\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003ePereira et al., American Journal of Physiology-Endocrinology and Metabolism 279:E570-E576 (2000).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1016\/S0301-472X(00)00160-0\" rel=\"noopener\" target=\"_blank\"\u003eProliferation kinetics and differentiation potential of ex vivo expanded human bone marrow stromal cells: implications for their use in cell therapy\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eBanfi et al., Experimental Hematology (2000).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1677\/joe.0.1650443\" rel=\"noopener\" target=\"_blank\"\u003ePlacental lactogen-I gene activation in differentiating trophoblast cells: extrinsic and intrinsic regulation involving mitogen-activated protein kinase signaling pathways\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003ePeters et al., Journal of Endocrinology 165:443-456 (2000).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1074\/jbc.275.18.13677\" rel=\"noopener\" target=\"_blank\"\u003eRas\/MEK\/ERK Up-regulation of the Fibroblast KCa Channel FIK Is a Common Mechanism for Basic Fibroblast Growth Factor and Transforming Growth Factor-β Suppression of Myogenesis\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003ePeña et al., Journal of Biological Chemistry 275:13677-13682 (2000).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1242\/jcs.113.7.1161\" rel=\"noopener\" target=\"_blank\"\u003eClonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eMuraglia et al., Journal of Cell Science 113:1161-1166 (2000).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1172\/JCI3459\" rel=\"noopener\" target=\"_blank\"\u003eBone morphogenetic proteins induce the expression of noggin, which limits their activity in cultured rat osteoblasts\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eGazzerro et al., Journal of Clinical Investigation 102:2106-2114 (1998).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1016\/S8756-3282(98)00009-X\" rel=\"noopener\" target=\"_blank\"\u003eA Nude Mouse Model for Human Bone Formation in Unloaded Conditions\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eMuraglia et al., Bone (1998).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1002\/(SICI)1096-9861(19980511)394:3%3C386::AID-CNE9%3E3.0.CO;2-Y\" rel=\"noopener\" target=\"_blank\"\u003eInsulin-related growth factors stimulate proliferation of retinal progenitors in the goldfish\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eBoucher and Hitchcock, Journal of Comparative Neurology 394:386-394 (1998).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1210\/endo.139.4.5950\" rel=\"noopener\" target=\"_blank\"\u003eMitogen-Activated Protein Kinase Kinase (MEK) Activity Is Required for Inhibition of Skeletal Muscle Differentiation by Insulin-Like Growth Factor 1 or Fibroblast Growth Factor 2\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eWeyman and Wolfman, Endocrinology 139:1794-1800 (1998).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1152\/ajpcell.1998.274.2.C472\" rel=\"noopener\" target=\"_blank\"\u003eEts-1 is an early response gene activated by ET-1 and PDGF-BB in vascular smooth muscle cells\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eNaito et al., American Journal of Physiology-Cell Physiology 274:C472-C480 (1998).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1210\/endo.138.10.5425\" rel=\"noopener\" target=\"_blank\"\u003eFibroblast growth factor-2 supports ex vivo expansion and maintenance of osteogenic precursors from human bone marrow\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eMartin et al., Endocrinology 138:4456-4462 (1997).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1038\/bjc.1996.496\" rel=\"noopener\" target=\"_blank\"\u003eDifferential responses of scirrhous and well-differentiated gastric cancer cells to orthotopic fibroblasts\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eYashiro et al., British Journal of Cancer 74:1096-1103 (1996).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1002\/(SICI)1097-4652(199601)166:1%3C188::AID-JCP20%3E3.0.CO;2-A\" rel=\"noopener\" target=\"_blank\"\u003ePro-inflammatory cytokines downregulate platelet derived growth factor-α receptor gene expression in human osteoblastic cells\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eKöse et al., Journal of Cellular Physiology 166:188-197 (1996).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/section\u003e\n\u003c!-- AB_PUBLISHED_USE_END --\u003e\n\u003cp class=\"ab-datasheet-link\"\u003e\u003ca href=\"https:\/\/helix-chartreuse-ypja.squarespace.com\/s\/Austral_Biologicals_GF-030_Datasheet.pdf\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003eDownload technical datasheet (PDF)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Austral Biologicals","offers":[{"title":"10 µg","offer_id":52518874186030,"sku":"GF-030-3","price":50.0,"currency_code":"USD","in_stock":true},{"title":"100 µg","offer_id":52518874218798,"sku":"GF-030-5","price":275.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1006\/2846\/3918\/files\/austral-biologicals-gf-030-10ug.png?v=1788008668","url":"https:\/\/www.australbiologicals.com\/products\/gf-030","provider":"Austral Biologicals","version":"1.0","type":"link"}