{"product_id":"fm-130cy","title":"Anti-Infectious Pancreatic Necrosis Virus (IPNV) VP2 Protein, IgG Fraction, Monoclonal, Clone 1B3\/E10","description":"\u003cp\u003eMouse monoclonal antibody IgG fraction (clone 1B3\/E10) obtained by immunizing mice with purified IPN virus. The IgG fraction was purified using Protein G-Sepharose.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStability:\u003c\/strong\u003e Stable at least one year at -20°C. Avoid repeated freezing and thawing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological activity:\u003c\/strong\u003e This antibody is able to neutralize IPN virus and can be used in ELISA (1:5,000 dilution), Western blot (1:1,000 dilution), and indirect immunofluorescence (1:500). It recognizes a 54 kDa protein corresponding to VP2 protein. IPNV is a single-shelled, non-enveloped virus that belongs to the family Birnaviridae. Its genoma comprises a bisegmented double-stranded RNA, the segment B encodes VP1 and the segment A yields a polyprotein, pVP2-VP4-VP3 and a small protein (VP5) dispensable for viral replication. The polyprotein is autocatalytically cleaved by the endoprotease VP4 to release the structural proteins pVP2 (62 kDa) and VP3. pVP2 is processed during virus maturation into VP2 (54 kDa) constituting the outer capsid component. IPNV is responsible for infectious pancreatic necrosis, a contagious fish disease characterized by severe damage to the internal organs and tissues with a high mortality among salmonids.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Solution at 1.0 mg\/mL in PBS.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReferences:\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e1. Roberts, R. J. \u0026amp; Pearson MD. (2005) J. Fish Dis. 28, 383-390.\u003cbr\u003e2. Galloux, M. et al. (2004) J. Gen. Virol. 85, 2231-2236.\u003cbr\u003e3. Rodriguez Saint-Jean S. et al. (2003) 62, 113-165.\u003cbr\u003e4. Duncan, R. et al. (1991) Virology 181, 541-552.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- AB_PUBLISHED_USE_START --\u003e\n\u003csection class=\"ab-published-use ab-published-use-complete\"\u003e\n\u003ch3 id=\"ab-published-use-fm-130cy\"\u003ePublications using FM-130CY\u003c\/h3\u003e\n\u003cp\u003eThe following scientific publication report use of this Austral Biologicals product:\u003c\/p\u003e\n\u003col class=\"ab-publication-list ab-publication-list-complete\"\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/doi.org\/10.1038\/s41598-017-03036-w\" rel=\"noopener\" target=\"_blank\"\u003eInfectious pancreatic necrosis virus enters CHSE-214 cells via macropinocytosis\u003c\/a\u003e. \u003cspan class=\"ab-publication-citation\"\u003eLevican et al., Sci Rep 7(1):3068 (2017).\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/section\u003e\n\u003c!-- AB_PUBLISHED_USE_END --\u003e\n\u003cp class=\"ab-datasheet-link\"\u003e\u003ca href=\"https:\/\/helix-chartreuse-ypja.squarespace.com\/s\/Austral_Biologicals_FM-130CY_Datasheet.pdf\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003eDownload technical datasheet (PDF)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Austral Biologicals","offers":[{"title":"100 µg","offer_id":52518873956654,"sku":"FM-130CY-5","price":425.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1006\/2846\/3918\/files\/austral-biologicals-fm-130cy-100ug.png?v=1788008680","url":"https:\/\/www.australbiologicals.com\/products\/fm-130cy","provider":"Austral Biologicals","version":"1.0","type":"link"}