{"product_id":"fm-070az","title":"Anti-Flavobacterium Columnare, IgG fraction, Monoclonal, Clone 4H10\/F12","description":"\u003cp\u003eMouse monoclonal antibody IgG fraction (clone 4H10\/F12) obtained by immunizing mice with a Chilean isolate of Flavobacterium columnare (whole bacterial cells). The IgG fraction was purified using Protein G-Sepharose.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStability:\u003c\/strong\u003e Stable at least one year at -20°C. Avoid repeated freezing and thawing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological activity:\u003c\/strong\u003e This antibody can be used in ELISA (1:1,000 dilution) and indirect immunofluorescence (1:1,000 dilution) assays. F.columnare is a yellow-pigmented filamentous Gram-negative bacterium and the causative agent of Columnaris disease, one of the most important bacterial diseases of freshwater fish. Columnaris disease is characterized as an acute to chronic infection of the gills, oropharynx, and skin with a high level of mortality (50-90%) in salmonid and non-salmonid species.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormulation:\u003c\/strong\u003e Solution at 1.0 mg\/mL in PBS.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReferences:\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e1. Thomas-Jinu, S. \u0026amp; Goodwin, A E. (2004) J. Fish Dis. 27, 29-35.\u003cbr\u003e2. Welker, T.L. et al. (2005) Dis. Aquat. Org. 63, 129-138.\u003cbr\u003e3. Zhang, Y. et al. (2006) J. Fish Dis. 29, 657-663.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- AB_PUBLISHED_USE_START --\u003e\n\u003csection class=\"ab-published-use\"\u003e\n\u003ch3 id=\"ab-published-use-fm-070az\"\u003ePublished product use\u003c\/h3\u003e\n\u003cp\u003eA peer-reviewed study reported use of FM-070AZ-5 for flow-cytometric detection of Flavobacterium covae:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e FM-070AZ-5 was fluorescein-labeled and used to identify and enumerate F. covae in water samples by flow cytometry. \u003ca href=\"https:\/\/doi.org\/10.3389\/fphys.2022.886480\" rel=\"noopener\" target=\"_blank\"\u003eKaimal et al., Frontiers in Physiology (2022)\u003c\/a\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- AB_PUBLISHED_USE_END --\u003e\n\u003cp class=\"ab-datasheet-link\"\u003e\u003ca href=\"https:\/\/helix-chartreuse-ypja.squarespace.com\/s\/Austral_Biologicals_FM-070AZ_Datasheet.pdf\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003eDownload technical datasheet (PDF)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Austral Biologicals","offers":[{"title":"100 µg","offer_id":52518873628974,"sku":"FM-070AZ-5","price":425.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1006\/2846\/3918\/files\/austral-biologicals-fm-070az-100ug.png?v=1788008690","url":"https:\/\/www.australbiologicals.com\/products\/fm-070az","provider":"Austral Biologicals","version":"1.0","type":"link"}