Rabbit Anti-Apple Mosaic Virus (ApMV) IgG fraction POLYCLONAL

IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a mixture of three synthetic peptides corresponding to aa7-17 (AIEVNGKWYSPK), aa61-73 (KSFPGEQWHEVSG), and aa140-151 (RTRVKAGKYSAK) of the ApMV coat protein. The IgG fraction was purified using Protein G-Sepharose.

• Storage: Store at -20°C.
• Stability: Stable at least one year at -20°C. Avoid repeated freezing and thawing.
• Biological activity: This polyclonal antibody reacts with extracts of ApMV-infected plants in PTA (Plate Trapped Antigen)-ELISA and DAS-ELISA (1: 200 dilution). ApMV belongs to the genus Ilarvirus of the family Bromoviridae and its genome consists of three segments of positive single-stranded RNA molecules. The coat protein (23 kDa) is characterized by a high variability in different isolates. ApMV is graft-transmissible and persists in propagative material.
• Formulation: Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
• References: Petrzik, K. (2005) Eur. J. Plant Pathol. 111, 335-360.
  Petrzik, K. & Lenz, O. (2002) Arch. Virol. 147, 1275-1285.
  Shiel, P. J. & Berger, P.H. (2000) J. Gen. Virol. 81, 273-278.
  Alrefai, R.H. et al. (1994) J. Gen. Virol. 75, 2847-2850.

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