Rabbit Anti-Raspberry Bushy Dwarf Virus (RBDV) IgG fraction POLYCLONAL

IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a mixture of three synthetic peptides corresponding to aa11-24 (KAQYELYNRKL NRA), aa149-161 (TFNDLAAQKAGDK), and aa202-212 (EAINLVSGLNK) of the RBDV coat protein. The IgG fraction was purified using Protein G-Sepharose.

• Storage: Store at -20°C.
• Stability: Stable at least one year at -20°C. Avoid repeated freezing and thawing.
• Biological activity: This polyclonal antibody reacts with extracts of RBDV infected plants in PTA (Plate-Trapped Antigen)-ELISA (1: 200 dilution) and it recognizes a band (30 kDa) correponding to RBDV coat protein by Western Blot. RBDV belongs to the genus Idaeovirus,its genome consists of two segments of positive single-stranded RNA molecules. The genome is packaged in isometric particles with a coat protein of 30 kDa. RBDV infects wild and cultivated Rubus species and is transmitted by pollen and seeds.
• Formulation: Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
• References: MacFarlane, S. & McGavin, W. (2009) J. Gen. Virol. 90, 747-753.
  Natsuaki, T. et al. (1991) J. Gen. Virol. 72, 2183-2189.
  Jones, A. T. et al. (2000) Eur. J. Plant Pathol. 106, 623-632.

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