Rabbit Anti-Prunus Necrotic Ringspot Virus (PNRSV) IgG fraction POLYCLONAL

IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a mixture of three synthetic peptides corresponding to aa18-34 (KRCHPNDALV PLRAQQR), aa 08-118 (QLMGQNLTLLT), and aa165-177 (EPPSDLDFDTFAR) of the PNRSV coat protein. The IgG fraction was purified using Protein G-Sepharose.

• Storage: Store at -20°C.
• Stability: Stable at least one year at -20°C. Avoid repeated freezing and thawing.
• Biological activity: This polyclonal antibody reacts with extracts of PNRSV-infected plants in PTA (Plate-Trapped Antigen)-ELISA (1: 50 dilution). PNRSV belongs to the genus Ilarvirus of the family Bromoviridae, its genome consists of the three segments of positive single-stranded RNA molecules. The PNRSV coat protein (25 kDa) is required for RNA accumulation, encapsidation, cell-to-cell movement, and systemic spread of the virus. PNRSV is a isometric virus and is transmitted by grafting and seeds.
• Formulation: Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
• References: Fiore, N. et al. (2008) Arch. Virol. 153, 909-919.
  Codoñer, F.M. et al.(2006) J. Gen. Virol. 80, 5833-5840.
  Aparicio, F. et al. (2003) Virology 313, 213-223.

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