AUSTRAL Biologicals Catalog

Product Sheet: Human transforming growth factor beta-1 (TGF beta-1) recombinant

Catalog NumberGF-230-2
Product IdGF-230-2
Product NameHuman transforming growth factor beta-1 (TGF beta-1) recombinant
CategoryGrowth Factors, Cytokines, Receptors and their Antibodies
SubcategoryGrowth Factors/Cytokines/Enzymes
DescriptionHuman Transforming Growth Factor beta1 (TGF- beta1 ) is stimulatory for cells of mesenchymal origin and inhibitory for cells of epithelial or neuroectodermal origin. Mature TGF- beta1 is a disulfide-linked homodimer of two chains composed of 112 amino acid residues each. There is a 70% homology between human TGF- beta1 and human TGF- beta2. Human TGF- beta1 is produced in genetically engineered bacteria, and purified by sequential chromatography.
Mol Weight25 KDa (dimeric protein)
PurityOver 98% pure by non-reduced SDS gel electrophoresis.
StorageStore at +4°C.
StabilitySeveral months at +4°C.
Biological ActivityThe biological activity of human TGF-&# beta1 is determined by measuring the acidification of the culture medium (Rat-1 cells) exposed to the growth factor.
Formulation25 µl (0.20 mg/ml) in 5 mM HCl (pH 2.5). Some of the liquid in the vial could have been evaporated with changes in the final volume. However, the 5µg of the protein are still inside the vial. We recommend, in order to control the proper concentration, to dilute the entire content to a known volume with 5mM HCl. Do not freeze this material.
References1. Ichijo, H. et al.(1994) The Journal of Biological Chemistry, Vol 266, No. 33, May, 22459-22464
2. Yan, Z., Winawer, S., and Friedman, E., (1994) The Journal of Biological Chemistry, Vol 269, No. 18, May, 13231-13237.
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

top | home