AUSTRAL Biologicals Catalog

Product Sheet: Tumor Necrosis Factor (TNF alpha) human (RECOMBINANT)

Catalog NumberGF-090-3
Product IdGF-090-3
Product NameTumor Necrosis Factor (TNF alpha) human (RECOMBINANT)
CategoryGrowth Factors, Cytokines, Receptors and their Antibodies
SubcategoryGrowth Factors/Cytokines/Enzymes
DescriptionHuman Tumor necrosis factor (TNF- alpha), also known as Cachectin, is a cytokine composed of 157 amino acid residues, having a molecular weight of 17 kDa (monomer). There is 79% homology between human and murine TNF- alpha, including conserved cysteine residues which form an intrachain disulfide bridge. Biological effects of this cytokine include antitumor activity in vivo and in vitro, activation of polymorphonuclear leukocytes, antiviral activity, and the induction of the release if Interleukin-1 or colony stimulating factors from a variety of sources. Human TNF- alpha (Cachectin) is produced in genetically engineered yeast, and purified by sequential chromatography.
Mol Weight17 KDa
PurityOver 95% pure by SDS gel electrophoresis.
StorageStore at -80°C.
StabilityAt least 1 year at -80°C.
Biological ActivityThe cell cytolytic activity is determined in the L929 cells (3 x 104 cells/assay) and it is 2-4 x 108 U/mg.
FormulationAt concentration of 1 mg/ml in 0.02M Tris-HCl/ 0.15 M NaCl (pH 8.0)
References1. Feinstein, R., Kanety, H., Papa, M.Z., lunenfeld, B., and Karasik, A., (1993) The Journal of Biological Chemistry, Vol 268, No. 35, December, 26055-26058
2. Kalthoff, H., Roeder, C., Brockhaus, M., Thiele, H.G., and Schmiegel, W., (1993) The Journal of Biological Chemistry, Vol 268, No. 4, February, 2762-2766 3. Chaturvedi, M.M. et al. (1994). J. Biol Chem. 269, 14575-14583.
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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