AUSTRAL Biologicals Catalog

Product Sheet: Platelet-derived Growth Factor (PDGF-BB) human (RECOMBINANT)

Catalog NumberGF-070-5
Product IdGF-070-5
Product NamePlatelet-derived Growth Factor (PDGF-BB) human (RECOMBINANT)
CategoryGrowth Factors, Cytokines, Receptors and their Antibodies
SubcategoryGrowth Factors/Cytokines/Enzymes
DescriptionHuman PDGF-BB homodimer is produced by genetically engineered yeast, and purified by sequential chromatography and ionic exchange HPLC. This polypeptide is composed of two identical polypeptide chains attached by disulfide bonds. As it has been described in the natural product, PDGF-BB is partially clipped at Arg32-Thr33. This cleavage is visible upon reduction of the polypeptide with a reducing agent. Note: this clipping does not affect the biological activity.
Mol WeightN/A
PurityOver 97% pure by N-terminal amino acid sequencing, amino acid composition, HPLC analysis, and SDS gel electrophoresis.
StorageStore at +4°C. Reconstitution is best in water or buffer near neutral pH.
StabilitySeveral months at +4°C. In solution, at pH 4-7, is stable for 2-3 months at -20°C.
Biological ActivityThe material is fully active as determined by its mitogenic activity measured by stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells.
FormulationLyophilized powder
References1. Ross, R. et al. (1986) Cell 46:155
2. Johnson, A. et al. (1984) EMBO J.3:921
3. Waterfield, M.D. et al. (1983) Nature 303: 35
4. Brake, A.J. et al. (1884) Proc. Natl. Acad. Sci. (USA) 81:4642
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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