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AUSTRAL Biologicals Catalog

Product Sheet: Acidic Fibroblast Growth Factor (FGF 1) human (RECOMBINANT)

Catalog NumberGF-040-4
Product IdGF-040-4
Product NameAcidic Fibroblast Growth Factor (FGF 1) human (RECOMBINANT)
Temperature-80°C
Unit50µg
Price300
AvailabilityAvailable
CategoryGrowth Factors, Cytokines, Receptors and their Antibodies
SubcategoryGrowth Factors/Cytokines/Enzymes
DescriptionAcidic FGF is a mitogen for cells of mesodermal and neuroectodermal origin, including fibroblasts, endothelial cells, astrocytes, neuroblasts, osteoblasts, and smooth muscle cells. This protein is composed of 140 amino acid residues, and has a 55% homology with basic FGF, including two conserved cysteine residues. Human Acidic FGF is produced by genetically engineered yeast, and purified by heparin-Sepharose affinity chromatography.
IsotypeN/A
Mol Weight15.5 KDa
PurityOver 95% pure by HPLC analysis and SDS gel electrophoresis.
StorageStore at -80°C. It is also possible to keep this protein at low pH (2-5) for a few days.
StabilityUnstable without a carrier protein like gelatin or bovine serum albumin. It is recommended to add some of these proteins, prior to aliquoting, for longer storage.
Biological ActivityHuman acidic FGF is mitogenic for a variety of normal cells in culture including fibroblasts, glial cells, vascular endothelial cells.
Formulation10 mM Tris-HCl, 1.5 M NaCl, pH 7.0 Concentration: 0.50 mg protein/ml.
References1. Gimenez-Gallego et al., Biochem. Biophys. Res. Comm.(1986) 138:611
2. Thornton et. al. (1983), Science 222, 623
3. Gospadorowicz, D. (1987) Methods Enzymol. 147:106
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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