AUSTRAL Biologicals Catalog

Product Sheet: Epidermal Growth Factor (EGF) human (RECOMBINANT)

Catalog NumberGF-010-8
Product IdGF-010-8
Product NameEpidermal Growth Factor (EGF) human (RECOMBINANT)
CategoryGrowth Factors, Cytokines, Receptors and their Antibodies
SubcategoryGrowth Factors/Cytokines/Enzymes
DescriptionHuman EGF is produced by genetically engineered yeast, and purified by sequential chromatography and reverse-phase HPLC. This polypeptide is identical to gamma-urogastrone, or equivalent to human EGF1-52.
Mol WeightN/A
PurityOver 97% pure by N-terminal amino acid sequencing, amino acid composition, HPLC analysis, and SDS gel electrophoresis.
StorageStore at +4°C. Reconstitution is best in water or buffer near neutral pH.
StabilitySeveral months at +4°C. In solution, at pH 4-7, is stable for 2-3 months at -20°C.
Biological ActivityThe mitogenic activity of human EGF is measured by the stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells. In addition, EGF activity has been determined by a receptor-binding assay (RBA) using A-431 cells.
FormulationLyophilized powder
References1. Cohen, S. (1962) J, Biol. Chem. 237:1555
2. Gregory, H. (1985) J.Cell Science Supp.. 3:11
3.- George-Nascimento, C. et al. (1988) Biochemistry 27:797-802
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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