AUSTRAL Biologicals Catalog

Product Sheet: Rabbit Anti-Prunus Necrotic Ringspot Virus (PNRSV) IgG fraction POLYCLONAL

Catalog NumberAP-2103-9
Product NameRabbit Anti-Prunus Necrotic Ringspot Virus (PNRSV) IgG fraction POLYCLONAL
CategoryInfectious Agents
SubcategoryPlant Pathogens (Agriculture)
DescriptionIgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a mixture of three synthetic peptides corresponding to aa18-34 (KRCHPNDALV PLRAQQR), aa 08-118 (QLMGQNLTLLT), and aa165-177 (EPPSDLDFDTFAR) of the PNRSV coat protein. The IgG fraction was purified using Protein G-Sepharose.
Mol WeightN/A
StorageStore at -20ºC.
StabilityStable at least one year at -20ºC. Avoid repeated freezing and thawing.
Biological ActivityThis polyclonal antibody reacts with extracts of PNRSV-infected plants in PTA (Plate-Trapped Antigen)-ELISA (1: 50 dilution). PNRSV belongs to the genus Ilarvirus of the family Bromoviridae, its genome consists of the three segments of positive single-stranded RNA molecules. The PNRSV coat protein (25 kDa) is required for RNA accumulation, encapsidation, cell-to-cell movement, and systemic spread of the virus. PNRSV is a isometric virus and is transmitted by grafting and seeds.
FormulationSolution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
ReferencesFiore, N. et. al. (2008) Arch. Virol. 153, 909-919.
Codoñer, F.M. et. al.(2006) J. Gen. Virol. 80, 5833-5840.
Aparicio, F. et. al. (2003) Virology 313, 213-223.
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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