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AUSTRAL Biologicals Catalog

Product Sheet: Rabbit Anti-Grapevine Leafroll-Associated Virus 1 (GLRaV-1) Coat Protein (petides) IgG fraction POLYCLONAL

Catalog NumberAP-1403-9
Product NameRabbit Anti-Grapevine Leafroll-Associated Virus 1 (GLRaV-1) Coat Protein (petides) IgG fraction POLYCLONAL
CategoryInfectious Agents
SubcategoryPlant Pathogens (Agriculture)
DescriptionIgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a mix of six synthetic peptides corresponding to aa6-19 (SQNDDDYNVVRSGNK), aa88-100 (SRPSTGQRFGDSEK), aa103-115 (RREMGDKLKRTFN), aa20-220 (YSNGERKLTYEKVK), aa259-261 (NEKVAMQHGVPKR), and aa310-322 (SSVL RNTGELKV) of coat protein from GLRaV-1. The IgG fraction was purified using Protein G-Sepharose.
Mol WeightN/A
StorageStore at -20ºC.
StabilityStable at least one year at -20ºC.
Biological ActivityThis polyclonal antibody reacts with extracts of GLRaV-1-infected plants in ELISA, PTA (Plate-Trapped Antigen)-ELISA, and Western blot (1: 200 dilution). GLRaV-1 belongs to the genus Ampelovirus of the family Closteroviridae. Its genome consists of a single-stranded positive-sense RNA and is encapsidated in filamentous particles containing a 35 kDa coat protein. GLRaV-1 is transmitted by vegetative propagation, grafting, scale insect, and mealybugs. It has been reported that GLRaV-1 has a great potential for genetic variation.
FormulationSolution at 1 mg/mL in PBS. Avoid repeated freezing and thawing. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
ReferencesAlabi, O.J. et. al. (2011) Phytopathology 101, 1446-1456.
Lanza, M. et. al. (2010) Trop. Plant Pathol. 35, 377-380.
Little, A. et. al. (2001) Virus Res. 80, 109-116.
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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