AUSTRAL Biologicals Catalog

Product Sheet: Rabbit anti-Alfalfa Mosaic Virus (AMV) IgG fraction POLYCLONAL

Catalog NumberAP-30103-9
Product NameRabbit anti-Alfalfa Mosaic Virus (AMV) IgG fraction POLYCLONAL
CategoryInfectious Agents
SubcategoryPlant Pathogens (Agriculture)
DescriptionRabbit polyclonal antibody IgG fraction obtained by immunizing rabbits with a mix of two synthetic peptides corresponding to aa14-27 (KPTKRSQNYAALRK), and aa193-205 (ERKAFPRAGPLRS) of coat protein from AMV. The IgG fraction was purified using Protein G-Sepharose.
Mol WeightN/A
StorageStore at -20ºC.
StabilityStable at least one year at -20ºC. Avoid repeated freezing and thawing.
Biological ActivityThis polyclonal antibody reacts with extracts of AMV-infected plants in PTA (Plate-Trapped Antigen)-ELISA and Western Blot assays (1: 50 dilution). AMV is a positive-strand RNA virus with tripartite genome belonging to the family Bromoviridae. It forms bacilliform or spherical particles and is transmitted in a non-persistent manner by numerous aphids species. The AMV genome is encapsidated by a single coat protein (24 kDa) which plays a key role in early and late AMV infection, functioning in genome activation, RNA replication, and in cell-to-cell systemic movement of virus.
FormulationSolution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
ReferencesHerranz, M.C. et. al. (2012) Mol. Plant Microbe Interact. 25, 1093-1103.
Bol, J.F. (2005) Annu. Rev. Phytopathol. 43, 39 - 62.
Bol, J.F (2003) Mol. Plant Pathol. 4, 1 - 8.
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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