AUSTRAL Biologicals Catalog

Product Sheet: Anti-Helicobacter pylori Flagellin A IgG fraction monoclonal antibody

Catalog NumberHPM-5041AZ-5
Product NameAnti-Helicobacter pylori Flagellin A IgG fraction monoclonal antibody
CategoryInfectious Agents
SubcategoryHelicobacter pylori
DescriptionMouse monoclonal antibody IgG fraction (clone 6G5/F6) obtained by immunizing mice with highly purified recombinant H. pylori flagellin A (FLaA) produced in genetically engineered E.coli cells. The IgG fraction was purified using Protein G-Sepharose.
StorageStore at -20ºC.
StabilityStable at least one year at -20ºC. Avoid repeated freezing and thawing.
Biological ActivityThis antibody recognizes specifically recombinant H. pylori FlaA, it does NOT cross react with purified recombinant H. pylori FlaB. It can be used in ELISA assay (1: 200 dilution) and Western blot (1: 4,000 dilution). H. pylori Flagellin is composed of two subunits FlaA (52 kDa) and FlaB (54 kDa), FlaA is the major component of flagellar filaments. Both FlaA and FlaB are responsible for bacterial motility and they are required for bacterial colonization and H. pylori survival in the stomach mucus.
FormulationSolution at 1.0 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
ReferencesOpazo, P. et al. (1999) APMIS 107, 1069-1078.
Müller, I. et al. (2002) Biol. Res. 35, 67-84.
Kostzynska, M. et al. (1991) J. Bacteriol. 173, 937-946.
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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