AUSTRAL Biologicals Catalog

Product Sheet: Anti-Nucleoprotein of Dobrava Virus (DOBV). IgG fraction monoclonal antibody. Clone 3A1/H9

Catalog NumberHNM-6031BZ1-5
Product NameAnti-Nucleoprotein of Dobrava Virus (DOBV). IgG fraction monoclonal antibody. Clone 3A1/H9
CategoryInfectious Agents
DescriptionMouse monoclonal antibody IgG fraction (clone 3A1/H9) obtained by immunizing mice with purified recombinant DOBV Nucleoprotein. The IgG fraction was purified using Protein G-Sepharose.
Mol WeightN/A
StorageStore at -20ºC.
StabilityStable at least one year at -20ºC. Avoid repeated freezing and thawing.
Biological ActivityThis antibody can be used in ELISA (1:30,000 dilution) and Western blot (1:15,000 dilution) analysis. It recognizes the nucleoprotein of DOBV and it has cross reactivity with nucleoproteins of Seoul Virus (SEOV) and Hantaan Virus (HTNV). Hantaviruses (family Bunyaviridae) are enveloped viruses that contain a tripartite, negative-stranded RNA genome encoding an RNA-dependent RNA polymerase (L), two integral membrane glycoproteins (G1 and G2), and a nucleocapsid (N) protein. The N protein is the most abundant viral component and the major antigen in early serological response in humans and mice. It has essential functions in viral RNA replication, encapsidation, and also in virus assembly. DOBV causes hemorrhagic fever with renal syndrome in central Europe and is carried by two different rodent species, Apodemus flavicollis and Apodemus agrarius.
FormulationSolution at 1.0 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
ReferencesKlempa, B. et al. (2004) J. Clin. Microbiol. 42, 1322-1325.
Vapalahti, O. et al (2003) Lancet Infect. Dis. 3, 653-661.
Sibold, C. et al (2001) J. Med. Virol. 63, 158-167.
Avsic-Zupanc, T. et al. (1995) J. Gen. Virol. 76, 2801-2808.
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

top | home