AUSTRAL Biologicals Catalog

Product Sheet: Rabbit anti-Human SHPRH IgG fraction polyclonal antibody

Catalog NumberPT-5025a-9
Product IdPT-5025a-9
Product NameRabbit anti-Human SHPRH IgG fraction polyclonal antibody
CategoryGrowth Factors, Cytokines, Receptors and their Antibodies
SubcategoryPolyclonal Antibodies (Cancer/Transcription Factors)
DescriptionIgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (DKTKKQAVGSPRKIQKETRK) corresponding to amino acids 521-540 of human SHPRH. The IgG fraction was purified using Protein G-Sepharose.
Mol WeightN/A
StorageStore at -20ºC.
StabilityStable at least one year at -20ºC. Avoid repeated freezing and thawing.
Biological ActivityThis polyclonal antibody reacts in ELISA and Western blot (1:500 dilution) using the immunizing peptide. SHPRH is a member of the SWI/SNF family of ATPaes/helicases, it posesses a C3HC4 RING motif characteristic of ubiquitin ligase protein and it has a fundamental role in DNA repair mechanism. Upon genotoxic stress, SHPRH associates with PCNA, RAD18, and the ubiquitin-conjugating enzyme UBC(E2) and promotes methyl methanesulfonate (MMS)-induced PCNA polyubiquitation suppressing genomic instability. SHPRH gene is mutated in a number of cancer cell lines, including those from melanomas and ovarian cancer.
FormulationSolution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
ReferencesSood, R. et al. (2003) Genomics 82, 153-161. Unk, I. et al. (2006) Proc. Natl. Acad. Sci 103, 18107-18112. Motegi, A. et al. (2006) J. Cell Biol. 175, 703-708.
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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