AUSTRAL Biologicals Catalog

Product Sheet: Rabbit anti-Human Poly[ADP-ribose] Polymerase-1 (PARP) IgG fraction polyclonal antibody

Catalog NumberPT-5020a-9
Product IdPT-5020a-9
Product NameRabbit anti-Human Poly[ADP-ribose] Polymerase-1 (PARP) IgG fraction polyclonal antibody
CategoryGrowth Factors, Cytokines, Receptors and their Antibodies
SubcategoryPolyclonal Antibodies (Cancer/Transcription Factors)
DescriptionIgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (GVDEVAKKKSKK) corresponding to amino acids 214-225 of human PARP. The IgG fraction was purified using Protein G-Sepharose.
Mol WeightN/A
StorageStore at -20ºC.
StabilityStable at least one year at -20ºC. Avoid repeated freezing and thawing.
Biological ActivityThis polyclonal antibody can be used in immunocytochemistry (1:100 dilution) and it recognizes PARP (115 kDa) by Western blot assays (1: 1000 dilution). Poly [ADP-ribose] polymerase-1 (PARP) is a nuclear protein that catalyses the poly ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. It is activated by DNA strand breaks and plays a role in DNA-base excision repair, DNA-damage signalling, transcription regulation and proteosomal function. PARP is cleaved by caspases during apoptosis to yield two apoptotic fragments, the amino-terminal DNA binding domain (25 kDa) and the carboxy-terminal catalytic domain (85 kDa). PARP cleavage facilitates the cellular disassembly and serves as a marker of cell undergoing apoptosis.
FormulationSolution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
ReferencesCherney, B.W. et al. (1987) Proc. Natl. Acad. Sci. USA 84, 8370-8374. Olivier, F.J. et al. (1999) Am. J. Hum. Genet. 64, 1282-1288. Kim, M.Y. et al. (2005) Genes & Dev. 19, 1951-1967. Schreiber, V. et al. (2006) Nat. Rev. Mol. Cell Biol. 7, 517-528.
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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