AUSTRAL Biologicals Catalog

Product Sheet: Rabbit anti-Human N-Myc IgG fraction polyclonal antibody

Catalog NumberPT-5001a-9
Product IdPT-5001a-9
Product NameRabbit anti-Human N-Myc IgG fraction polyclonal antibody
CategoryGrowth Factors, Cytokines, Receptors and their Antibodies
SubcategoryPolyclonal Antibodies (Cancer/Transcription Factors)
DescriptionIgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (SPYVESEDAPPQKK) corresponding to amino acids 337-349 of human N-Myc. The IgG fraction was purified using Protein G-Sepharose.
Mol WeightN/A
StorageStore at -20ºC.
StabilityStable at least one year at -20ºC. Avoid repeated freezing and thawing.
Biological ActivityThis polyclonal antibody reacts in ELISA and Western blot assays (1: 500 dilution) using the immunizing peptide. N-Myc is a 49.5 kDa nuclear phosphoprotein that contains a basic helix-loop-helix leucine zipper domain involved in DNA binding and protein dimerization. It regulates gene expression by dimerizing with itself or other helix-loop-helix proteins and this complex binds to DNA E-box sequences of regulated genes. N-Myc is expressed in high levels during embryonic development and in the adult during B-cell differentiation. It is overexpressed in a variety of tumors, aminly neuroblastomas and is associated with rapid progression and poor prognosis.
FormulationSolution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
ReferencesSlamon, D.J. et al. (1986) Science 232, 768-772. Eisenman, R.N. (2001) Genes & Dev. 15, 2023-2030. Knoepfler, P.S. et al. (2006) EMBO J. 25, 2723-2734.
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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